PloS one, 2015-09, Vol.10 (9), p.e0137568-e0137568
2015
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- Autor(en) / Beteiligte
- Titel
- Detection of Hepatitis B Virus (HBV) Genomes and HBV Drug Resistant Variants by Deep Sequencing Analysis of HBV Genomes in Immune Cell Subsets of HBV Mono-Infected and/or Human Immunodeficiency Virus Type-1 (HIV-1) and HBV Co-Infected Individuals
- Ist Teil von
- PloS one, 2015-09, Vol.10 (9), p.e0137568-e0137568
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The hepatitis B virus (HBV) and the human immunodeficiency virus type 1 (HIV-1) can infect cells of the lymphatic system. It is unknown whether HIV-1 co-infection impacts infection of peripheral blood mononuclear cell (PBMC) subsets by the HBV. To compare the detection of HBV genomes and HBV sequences in unsorted PBMCs and subsets (i.e., CD4+ T, CD8+ T, CD14+ monocytes, CD19+ B, CD56+ NK cells) in HBV mono-infected vs. HBV/HIV-1 co-infected individuals. Total PBMC and subsets isolated from 14 HBV mono-infected (4/14 before and after anti-HBV therapy) and 6 HBV/HIV-1 co-infected individuals (5/6 consistently on dual active anti-HBV/HIV therapy) were tested for HBV genomes, including replication indicative HBV covalently closed circular (ccc)-DNA, by nested PCR/nucleic hybridization and/or quantitative PCR. In CD4+, and/or CD56+ subsets from two HBV monoinfected cases, the HBV polymerase/overlapping surface region was analyzed by next generation sequencing. All analyzed whole PBMC from HBV monoinfected and HBV/HIV coinfected individuals were HBV genome positive. Similarly, HBV DNA was detected in all target PBMC subsets regardless of antiviral therapy, but was absent from the CD4+ T cell subset from all HBV/HIV-1 positive cases (P<0.04). In the CD4+ and CD56+ subset of 2 HBV monoinfected cases on tenofovir therapy, mutations at residues associated with drug resistance and/or immune escape (i.e., G145R) were detected in a minor percentage of the population. HBV genomes and drug resistant variants were detectable in PBMC subsets from HBV mono-infected individuals. The HBV replicates in PBMC subsets of HBV/HIV-1 patients except the CD4+ T cell subpopulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0137568Titel-ID: cdi_plos_journals_1719289446
- Format
- –
- Schlagworte
- Acquired immune deficiency syndrome, Adult, AIDS, Antigens, Antiviral agents, Antiviral drugs, CD14 antigen, CD19 antigen, CD4 antigen, CD4-Positive T-Lymphocytes - virology, CD56 antigen, CD8 antigen, Circular DNA, Coinfection - complications, Coinfection - virology, Deoxyribonucleic acid, Development and progression, DNA, Dosage and administration, Drug resistance, Drug Resistance, Viral, Drug therapy, Female, Gastroenterology, Gene sequencing, Genome, Viral, Genomes, Hepatitis, Hepatitis B, Hepatitis B - complications, Hepatitis B - virology, Hepatitis B virus - genetics, Hepatitis B virus - isolation & purification, Hepatology, High-Throughput Nucleotide Sequencing, HIV, HIV infections, HIV Infections - complications, HIV Infections - virology, HIV-1 - isolation & purification, Human immunodeficiency virus, Humans, Hybridization, Immunology, Infections, Infectious diseases, Laboratories, Leukocytes, Mononuclear - virology, Liver cancer, Liver diseases, Lymphatic system, Lymphocytes B, Lymphocytes T, Male, Middle Aged, Monocytes, Mutation, Patient outcomes, Peripheral blood mononuclear cells, Plasma, Polymerase chain reaction, Target detection, Tenofovir, Therapy, Veterinary medicine, Viruses