Details
- Autor(en) / Beteiligte
- Titel
- Mature Erythrocytes of Iguana iguana (Squamata, Iguanidae) Possess Functional Mitochondria
- Ist Teil von
- PloS one, 2015-09, Vol.10 (9), p.e0136770-e0136770
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Electron microscopy analyses of Iguana iguana blood preparations revealed the presence of mitochondria within erythrocytes with well-structured cristae. Fluorescence microscopy analyses upon incubation with phalloidin-FITC, Hoechst 33342 and mitochondrial transmembrane potential (Δψm)-sensitive probe MitoTracker Red indicated that mitochondria i) widely occur in erythrocytes, ii) are polarized, and iii) seem to be preferentially confined at a "perinuclear" region, as confirmed by electron microscopy. The analysis of NADH-dependent oxygen consumption showed that red blood cells retain the capability to consume oxygen, thereby providing compelling evidence that mitochondria of Iguana erythrocytes are functional and capable to perform oxidative phosphorylation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0136770Titel-ID: cdi_plos_journals_1719285593
- Format
- –
- Schlagworte
- Animals, Blood, Blood cells, Cristae, Deoxyribonucleic acid, DNA, Electron microscopy, Erythrocytes, Erythrocytes - metabolism, Erythrocytes - ultrastructure, Fluorescence, Fluorescence microscopy, Hemoglobin, Iguana iguana, Iguanas - metabolism, Iguanidae, Incubation, Laboratories, Mammals, Membrane potential, Membrane Potential, Mitochondrial, Mitochondria, Mitochondria - metabolism, Mitochondria - ultrastructure, Mitochondrial DNA, NAD - metabolism, NADH, Nicotinamide adenine dinucleotide, Oxidative Phosphorylation, Oxidative stress, Oxygen, Oxygen consumption, Phalloidin, Phosphorylation, Red blood cells, Snakes, Squamata, Vertebrates