PloS one, 2015-07, Vol.10 (7), p.e0130792-e0130792
2015
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Details
- Autor(en) / Beteiligte
- Titel
- MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis
- Ist Teil von
- PloS one, 2015-07, Vol.10 (7), p.e0130792-e0130792
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0130792Titel-ID: cdi_plos_journals_1700107705
- Format
- –
- Schlagworte
- ABC transporters, Activation, Adenosine triphosphatase, Adenosine Triphosphatases - genetics, Adenosine Triphosphatases - metabolism, Animals, Antigens, ATP-Binding Cassette Transporters - genetics, ATP-Binding Cassette Transporters - metabolism, ATPases, Auditory defects, Bacteria, Bacterial infections, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Biochemistry, Carbohydrate Metabolism, Carbohydrates, Cell division, Cloning, Colonization, Deletion mutant, E coli, Escherichia coli, Female, Gene Deletion, Gene expression, Glucose, Glycogen, Gram-positive bacteria, Health aspects, Hogs, Infection, Kinases, Laboratory animals, Maltotetraose, Maltotriose, Melibiose, Metabolism, Mice, Pathogenesis, Pathogens, Physiological aspects, Pigs, Raffinose, Small & medium sized enterprises-SME, Streptococcal Infections - enzymology, Streptococcal Infections - genetics, Streptococcus, Streptococcus infections, Streptococcus suis - enzymology, Streptococcus suis - pathogenicity, Stress response, Substrate Specificity - genetics, Suidae, Swine, Utilization, Veterinarians, Veterinary colleges, Veterinary medicine