PloS one, 2015-07, Vol.10 (7), p.e0132511-e0132511
2015
Details
- Autor(en) / Beteiligte
- Titel
- Sentinel Node Biopsy for the Head and Neck Using Contrast-Enhanced Ultrasonography Combined with Indocyanine Green Fluorescence in Animal Models: A Feasibility Study
- Ist Teil von
- PloS one, 2015-07, Vol.10 (7), p.e0132511-e0132511
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Sentinel node navigation surgery is gaining popularity in oral cancer. We assessed application of sentinel lymph node navigation surgery to pharyngeal and laryngeal cancers by evaluating the combination of contrast-enhanced ultrasonography and indocyanine green fluorescence in animal models. This was a prospective, nonrandomized, experimental study in rabbit and swine animal models. A mixture of indocyanine green and Sonazoid was used as the tracer. The tracer mixture was injected into the tongue, larynx, or pharynx. The sentinel lymph nodes were identified transcutaneously by infra-red camera and contrast-enhanced ultrasonography. Detection time and extraction time of the sentinel lymph nodes were measured. The safety of the tracer mixture in terms of mucosal reaction was evaluated macroscopically and microscopically. Sentinel lymph nodes were detected transcutaneously by contrast-enhanced ultrasonography alone. The number of sentinel lymph nodes detected was one or two. Despite observation of contrast enhancement of Sonazoid for at least 90 minutes, the number of sentinel lymph nodes detected did not change. The average extraction time of sentinel lymph nodes was 4.8 minutes. Indocyanine green fluorescence offered visual information during lymph node biopsy. The safety of the tracer was confirmed by absence of laryngeal edema both macro and microscopically. The combination method of indocyanine green fluorescence and contrast-enhanced ultrasonography for detecting sentinel lymph nodes during surgery for head and neck cancer seems promising, especially for pharyngeal and laryngeal cancer. Further clinical studies to confirm this are warranted.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0132511Titel-ID: cdi_plos_journals_1695377954
- Format
- –
- Schlagworte
- Animal models, Animals, Biopsy, Cancer, Change detection, Committees, Contrast Media, Disease Models, Animal, Edema, Feasibility Studies, Ferric Compounds, Fluorescence, Head, Head & neck cancer, Head and neck cancer, Head and Neck Neoplasms - diagnostic imaging, Head and Neck Neoplasms - pathology, Indocyanine Green - metabolism, Iron, Laboratory animals, Laryngeal cancer, Larynx, Livestock, Lymph nodes, Lymph Nodes - pathology, Lymph Nodes - ultrastructure, Lymphatic system, Male, Medical imaging, Medicine, Melanoma, Mucosa, Navigation behavior, NMR, Nuclear magnetic resonance, Oral cancer, Otolaryngology, Oxides, Particle size, Patients, Pharynx, Physicians, Rabbits, Radioisotopes, Reproducibility of Results, Safety, Sentinel Lymph Node Biopsy, Surgery, Sus scrofa, Swine, Time Factors, Tomography, Tongue, Tracers (Biology), Ultrasonic imaging, Ultrasonography, Ultrasound, Ultrasound imaging