PloS one, 2015-06, Vol.10 (6), p.e0131208
2015
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- Autor(en) / Beteiligte
- Titel
- Titanium Dioxide Nanoparticles Induce Endoplasmic Reticulum Stress-Mediated Autophagic Cell Death via Mitochondria-Associated Endoplasmic Reticulum Membrane Disruption in Normal Lung Cells
- Ist Teil von
- PloS one, 2015-06, Vol.10 (6), p.e0131208
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Nanomaterials are used in diverse fields including food, cosmetic, and medical industries. Titanium dioxide nanoparticles (TiO2-NP) are widely used, but their effects on biological systems and mechanism of toxicity have not been elucidated fully. Here, we report the toxicological mechanism of TiO2-NP in cell organelles. Human bronchial epithelial cells (16HBE14o-) were exposed to 50 and 100 μg/mL TiO2-NP for 24 and 48 h. Our results showed that TiO2-NP induced endoplasmic reticulum (ER) stress in the cells and disrupted the mitochondria-associated endoplasmic reticulum membranes (MAMs) and calcium ion balance, thereby increasing autophagy. In contrast, an inhibitor of ER stress, tauroursodeoxycholic acid (TUDCA), mitigated the cellular toxic response, suggesting that TiO2-NP promoted toxicity via ER stress. This novel mechanism of TiO2-NP toxicity in human bronchial epithelial cells suggests that further exhaustive research on the harmful effects of these nanoparticles in relevant organisms is needed for their safe application.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0131208Titel-ID: cdi_plos_journals_1695138693
- Format
- –
- Schlagworte
- Apoptosis, Autophagy, Autophagy - drug effects, Biochemistry, Biocompatibility, Biological effects, Calcium, Calcium (reticular), Calcium - metabolism, Calcium ions, Cell death, Cells, Cultured, Cosmetics industry, Endoplasmic reticulum, Endoplasmic Reticulum - metabolism, Endoplasmic Reticulum - ultrastructure, Endoplasmic Reticulum Stress - drug effects, Epithelial cells, Epithelial Cells - drug effects, Epithelial Cells - pathology, Food industry, Homeostasis, Homeostasis - drug effects, Humans, Intracellular Membranes - drug effects, Intracellular Membranes - metabolism, Intracellular Membranes - ultrastructure, Laboratories, Lung - pathology, Lungs, Membranes, Mitochondria, Mitochondria - metabolism, Mitochondria - ultrastructure, Mitochondrial DNA, Morphology, Nanomaterials, Nanoparticles, Nanoparticles - toxicity, Nanoparticles - ultrastructure, Nanotechnology, Organelles, Oxidative stress, Phagocytosis, Proteins, R&D, Reactive Oxygen Species - metabolism, Research & development, Science, Stresses, Surgical implants, Taurochenodeoxycholic Acid - pharmacology, Tauroursodeoxycholic acid, Titanium, Titanium - toxicity, Titanium dioxide, Toxicity, Toxicology, Veterinary colleges, Veterinary medicine