Details
- Autor(en) / Beteiligte
- Titel
- Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine
- Ist Teil von
- PloS one, 2015-06, Vol.10 (6), p.e0100476-e0100476
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution and annotated them regarding genes, transcription start sites (TSS) and CpG islands. We found that global DNA methylation decreased in vaccinated birds, presenting 704 hypomethylated and 451 hypermethylated DMRs, respectively. Additionally, we performed an enrichment analysis detecting gene networks, in which cancer and RNA post-transcriptional modification appeared in the first place, followed by humoral immune response, immunological disease and inflammatory disease. The top four identified canonical pathways were EIF2 signaling, regulation of EIF4 and p70S6K signaling, axonal guidance signaling and mTOR signaling, providing new insight regarding the mechanisms of ILT etiology. Lastly, the association between DNA methylation and differentially expressed genes was examined, and detected negative correlation in seventeen of the eighteen genes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0100476Titel-ID: cdi_plos_journals_1691039796
- Format
- –
- Schlagworte
- Animals, Axon guidance, Bioinformatics, Birds, Cancer, Chickens, Chickens - genetics, Chickens - immunology, CpG islands, CpG Islands - genetics, Deoxyribonucleic acid, Disease, DNA, DNA methylation, DNA Methylation - genetics, DNA Methylation - immunology, DNA vaccines, Embryos, Epigenetics, Etiology, Eukaryotic Initiation Factor-2 - genetics, Eukaryotic Initiation Factor-2 - immunology, Gene expression, Gene Regulatory Networks - genetics, Gene Regulatory Networks - immunology, Gene sequencing, Genes, Genome - genetics, Genome - immunology, Genomes, Herpesvirus 1, Gallid - immunology, Immune response, Immune response (humoral), Immune system, Immune System Diseases - genetics, Immune System Diseases - immunology, Immunization, Immunology, Infections, Infectious diseases, Inflammation - genetics, Inflammation - immunology, Kinases, Laboratories, Laryngotracheitis, Post-transcription, Poultry, Ribonucleic acid, Ribosomal Protein S6 Kinases, 70-kDa - genetics, Ribosomal Protein S6 Kinases, 70-kDa - immunology, RNA, RNA modification, RNA Processing, Post-Transcriptional - genetics, RNA Processing, Post-Transcriptional - immunology, Signal Transduction - genetics, Signal Transduction - immunology, Signaling, TOR protein, TOR Serine-Threonine Kinases - genetics, TOR Serine-Threonine Kinases - immunology, Transcription Initiation Site - physiology, Vaccines, Vaccines - immunology, Veterinary colleges, Veterinary medicine