PLoS genetics, 2015-01, Vol.11 (1), p.e1004931-e1004931
2015
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- Autor(en) / Beteiligte
- Titel
- Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast
- Ist Teil von
- PLoS genetics, 2015-01, Vol.11 (1), p.e1004931-e1004931
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Elongator is a conserved protein complex comprising six different polypeptides that has been ascribed a wide range of functions, but which is now known to be required for modification of uridine residues in the wobble position of a subset of tRNAs in yeast, plants, worms and mammals. In previous work, we showed that Elongator's largest subunit (Elp1; also known as Iki3) was phosphorylated and implicated the yeast casein kinase I Hrr25 in Elongator function. Here we report identification of nine in vivo phosphorylation sites within Elp1 and show that four of these, clustered close to the Elp1 C-terminus and adjacent to a region that binds tRNA, are important for Elongator's tRNA modification function. Hrr25 protein kinase directly modifies Elp1 on two sites (Ser-1198 and Ser-1202) and through analyzing non-phosphorylatable (alanine) and acidic, phosphomimic substitutions at Ser-1198, Ser-1202 and Ser-1209, we provide evidence that phosphorylation plays a positive role in the tRNA modification function of Elongator and may regulate the interaction of Elongator both with its accessory protein Kti12 and with Hrr25 kinase.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1553-7404, 1553-7390eISSN: 1553-7404DOI: 10.1371/journal.pgen.1004931Titel-ID: cdi_plos_journals_1685137654
- Format
- –
- Schlagworte
- Adaptor Proteins, Signal Transducing - genetics, Alanine - genetics, Biology and life sciences, Casein Kinase I - genetics, Casein Kinase I - metabolism, Colleges & universities, Gene Expression Regulation, Fungal, Genetic aspects, Histone Acetyltransferases - genetics, Histone Acetyltransferases - metabolism, Identification and classification, Kinases, Methods, Multiprotein Complexes - genetics, Mutation, Peptide Elongation Factors - genetics, Peptide Elongation Factors - metabolism, Phenotype, Phosphorylation, Physiological aspects, Plasmids, Polypeptides, Proteins, RNA polymerase, RNA sequencing, RNA, Transfer - genetics, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae Proteins - genetics, Saccharomyces cerevisiae Proteins - metabolism, Transfer RNA, Uridine - genetics, Yeast, Yeasts (Fungi)