PloS one, 2015-05, Vol.10 (5), p.e0127907-e0127907
2015
Details
- Autor(en) / Beteiligte
- Titel
- Immunogenicity without Efficacy of an Adenoviral Tuberculosis Vaccine in a Stringent Mouse Model for Immunotherapy during Treatment
- Ist Teil von
- PloS one, 2015-05, Vol.10 (5), p.e0127907-e0127907
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- To investigate if bacterial persistence during TB drug treatment could be overcome by modulation of host immunity, we adapted a clinically-relevant model developed for the evaluation of new drugs and examined if immunotherapy with two adenoviral vaccines, Ad35-TBS (AERAS-402) and Ad26-TBS, could shorten therapy in mice. Even though immunotherapy resulted in strong splenic IFN-γ responses, no effect on bacterial replication in the lungs was seen. Multiplex assay analysis of lung samples revealed the absence of cytokine augmentation such as IFN-γ, TNF-α and IL-2, suggesting that immunization failed to induce immunity in the lungs. In this model, we show that IFN-γ levels were not associated with protection against disease relapse. The results obtained from our study raise questions regarding the traits of protective TB immunity that are relevant for the development of future immunotherapeutic and post-exposure vaccination strategies.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0127907Titel-ID: cdi_plos_journals_1682423897
- Format
- –
- Schlagworte
- Adenoviridae - genetics, Animals, Antibiotics, Antigens, Augmentation, Bacteria, Bacterial Load, Care and treatment, Chemotherapy, Cytokines - metabolism, Diagnosis, Disease Models, Animal, Dosage and administration, Drug development, Drug therapy, Drugs, Female, Genetic Vectors - genetics, Immunity, Immunization, Immunogenicity, Immunotherapy, Infections, Infectious diseases, Interferon, Interleukin 2, Lung - immunology, Lung - metabolism, Lung - microbiology, Lung - pathology, Lungs, Medicine, Mice, Multiplexing, Mycobacterium tuberculosis, Mycobacterium tuberculosis - immunology, Risk factors, Spleen, Spleen - immunology, Substance abuse treatment, T-Lymphocyte Subsets - immunology, T-Lymphocyte Subsets - metabolism, Treatment Failure, Tuberculosis, Tuberculosis - immunology, Tuberculosis - microbiology, Tuberculosis - therapy, Tuberculosis vaccines, Tuberculosis Vaccines - genetics, Tuberculosis Vaccines - immunology, Tumor necrosis factor-α, Vaccines, γ-Interferon