PloS one, 2015-05, Vol.10 (5), p.e0119396-e0119396
2015
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- Autor(en) / Beteiligte
- Titel
- MicroRNA-21 Regulates PI3K/Akt/mTOR Signaling by Targeting TGFβI during Skeletal Muscle Development in Pigs
- Ist Teil von
- PloS one, 2015-05, Vol.10 (5), p.e0119396-e0119396
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- MicroRNAs (miRNAs), which are short (22-24 base pairs), non-coding RNAs, play critical roles in myogenesis. Using Solexa deep sequencing, we detected the expression levels of 229 and 209 miRNAs in swine skeletal muscle at 90 days post-coitus (E90) and 100 days postnatal (D100), respectively. A total of 138 miRNAs were up-regulated on E90, and 31 were up-regulated on D100. Of these, 9 miRNAs were selected for the validation of the small RNA libraries by quantitative RT-PCR (RT-qPCR). We found that miRNA-21 was down-regulated by 17-fold on D100 (P<0.001). Bioinformatics analysis suggested that the transforming growth factor beta-induced (TGFβI) gene was a potential target of miRNA-21. Both dual luciferase reporter assays and western blotting demonstrated that the TGFβI gene was regulated by miRNA-21. Co-expression analysis revealed that the mRNA expression levels of miRNA-21 and TGFβI were negatively correlated (r = -0.421, P = 0.026) in skeletal muscle during the 28 developmental stages. Our results revealed that more miRNAs are expressed in prenatal than in postnatal skeletal muscle. The miRNA-21 is a novel myogenic miRNA that is involved in skeletal muscle development and regulates PI3K/Akt/mTOR signaling by targeting the TGFβI gene.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0119396Titel-ID: cdi_plos_journals_1679402385
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, Agriculture, AKT protein, Animal sciences, Animals, Base pairs, Bioinformatics, Breeding of animals, Cells, Cultured, Developmental stages, Gene expression, Gene Expression Regulation, Developmental, Genomes, Germplasm, Growth factors, Hogs, Laboratories, Livestock, MicroRNAs, MicroRNAs - genetics, MicroRNAs - metabolism, miRNA, Molecular Sequence Data, Muscle, Skeletal - growth & development, Muscle, Skeletal - metabolism, Muscles, Musculoskeletal system, Myogenesis, Nutrition, Phosphatidylinositol 3-Kinases - metabolism, Polymerase chain reaction, Proto-Oncogene Proteins c-akt - metabolism, Pulmonary arteries, Ribonucleic acid, RNA, Sequence Analysis, RNA, Sexual intercourse, Signal Transduction, Signaling, Skeletal muscle, Smooth muscle, Studies, Suidae, Swine, TOR protein, TOR Serine-Threonine Kinases - metabolism, Transforming Growth Factor beta - genetics, Transforming Growth Factor beta - metabolism, Transforming growth factor-b, Western blotting, Zoology