Details
- Autor(en) / Beteiligte
- Titel
- Molecular Cloning and Characterisation of Farnesyl Pyrophosphate Synthase from Tripterygium wilfordii
- Ist Teil von
- PloS one, 2015-05, Vol.10 (5), p.e0125415-e0125415
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Farnesylpyrophosphate synthase (FPS) catalyzes the biosynthesis of farnesyl pyrophosphate (FPP), which is an important precursor of sesquiterpenoids such as artemisinin and wilfordine. In the present study, we report the molecular cloning and characterization of two full-length cDNAs encoding FPSs from Tripterygium wilfordii (TwFPSs). TwFPSs maintained their capability to synthesise FPP in vitro when purified as recombinant proteins from E. coli. Consistent with the endogenous role of FPS in FPP biosynthesis, TwFPSs were highly expressed in T. wilfordii roots, and were up-regulated upon methyl jasmonate (MeJA) treatment. The global gene expression profiles suggested that the TwFPSs might play an important regulatory role interpenoid biosynthesis in T. wilfordii, laying the groundwork for the future study of the synthetic biology of natural terpene products.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0125415Titel-ID: cdi_plos_journals_1678577047
- Format
- –
- Schlagworte
- Acetates - pharmacology, Amino Acid Sequence, Arabidopsis thaliana, Artemisia annua, Artemisinin, Biocatalysis - drug effects, Biosynthesis, Chinese medicine, Cloning, Cloning, Molecular, Cyclopentanes - pharmacology, DNA, Complementary - genetics, E coli, Electrophoresis, Polyacrylamide Gel, Enzymes, Farnesyl pyrophosphate synthase, Gas Chromatography-Mass Spectrometry, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Plant - drug effects, Genetic aspects, Geranyltranstransferase - chemistry, Geranyltranstransferase - genetics, Geranyltranstransferase - metabolism, Herbal medicine, Jasmonic acid, Methyl jasmonate, Molecular Sequence Data, Observations, Organ Specificity - drug effects, Organ Specificity - genetics, Oxylipins - pharmacology, Phylogeny, Plant Proteins - chemistry, Plant Proteins - genetics, Plant Proteins - metabolism, Properties, Proteins, Recombinant Proteins - metabolism, Sesquiterpenoids, Sterols, Terpenes, Thunder god vine, Tripterygium - enzymology, Tripterygium - genetics