PloS one, 2015-04, Vol.10 (4), p.e0121296-e0121296
2015
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- Autor(en) / Beteiligte
- Titel
- Inhibitors of eicosanoid biosynthesis influencing the transcripts level of sHSP21.4 gene induced by pathogen infections, in Antheraea pernyi
- Ist Teil von
- PloS one, 2015-04, Vol.10 (4), p.e0121296-e0121296
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a sHsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 kDa protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus (NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0121296Titel-ID: cdi_plos_journals_1676336734
- Format
- –
- Schlagworte
- Analysis, Animals, Antheraea pernyi, Aromatic-L-amino-acid decarboxylase, Bacterial infections, Beauveria - physiology, Beauveria bassiana, Bioinformatics, Biosynthesis, Cell cycle, Ceratitis capitata, Cloning, Molecular, Dexamethasone - pharmacology, Dihydroxyphenylalanine, Double-stranded RNA, E coli, Eicosanoic Acids - metabolism, Eicosanoids, Escherichia coli - physiology, Fat body, Fungi, Gene expression, Gene Expression Regulation - drug effects, Health aspects, Heat shock proteins, Heat-Shock Proteins, Small - chemistry, Heat-Shock Proteins, Small - genetics, Heat-Shock Proteins, Small - metabolism, Immune response, Immune system, Immunity, Indomethacin - pharmacology, Infections, Inhibitors, Insect Proteins - chemistry, Insect Proteins - genetics, Insect Proteins - metabolism, Insects, Laboratories, Lepidoptera, Life sciences, Lipid Metabolism - drug effects, Lysozyme, Masoprocol - pharmacology, Microorganisms, Midgut, Moths - genetics, Moths - immunology, Moths - microbiology, Nucleopolyhedroviruses - physiology, Pathogens, Phylogenetics, Phylogeny, Protease inhibitors, Protein folding, Proteins, RNA, Messenger - metabolism, Schistocerca gregaria, Serine, Serine proteinase, Silk, Small heat shock proteins, Viruses