PloS one, 2015-04, Vol.10 (4), p.e0123575-e0123575
2015
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- Autor(en) / Beteiligte
- Titel
- Down-regulation of microRNA-223 promotes degranulation via the PI3K/Akt pathway by targeting IGF-1R in mast cells
- Ist Teil von
- PloS one, 2015-04, Vol.10 (4), p.e0123575-e0123575
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Mast cells play a central role in allergic and inflammatory disorders by inducing degranulation and inflammatory mediator release. Recent reports have shown that miRNAs play an important role in inflammatory response regulation. Therefore, the role of miR-223 in mast cells was investigated. The expression of miR-223 was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) in immunoglobulin E (IgE)-mediated mast cells. After successful miR-223 inhibition by transfection, degranulation was detected in IgE-mediated mast cells. The phosphorylation of IκB-α and Akt were examined using western blotting. NF-κB was tested using electrophoretic mobility shift assay. PI3K-inhibitor (LY294002) was used to investigate whether the PI3K/Akt pathway was essential for mast cell activation. The TargetScan database and a luciferase reporter system were used to identify whether insulin-like growth factor 1 receptor (IGF-1R) is a direct target of miR-223. MiR-223 expression was up-regulated in IgE-mediated mast cells, whereas its down-regulation promoted mast cell degranulation. Levels of IκB-α and Akt phosphorylation as well as NF-κB were increased in miR-223 inhibitor cells. LY294002 could block the PI3K/Akt signaling pathway and rescue the promotion caused by suppressing miR-223 in mast cells. IGF-1R was identified as a direct target of miR-223. These findings suggest that down-regulation of miR-223 promotes degranulation via the PI3K/Akt pathway by targeting IGF-1R in mast cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0123575Titel-ID: cdi_plos_journals_1673122366
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, AKT protein, Allergies, Animals, Apoptosis, Asthma, Cell activation, Cell Degranulation - drug effects, Cell Line, Chromones - pharmacology, Degranulation, Dermatitis, Down-regulation, Down-Regulation - drug effects, Electrophoretic mobility, Enzymes, Families & family life, Gene expression, Histamine, Hospitals, Immunoglobulin E, Immunoglobulin E - metabolism, Infection, Inflammation, Inflammatory diseases, Inflammatory response, Inhibitors, Insulin, Insulin-like growth factors, Kinases, Luciferase, Mast cells, Mast Cells - drug effects, Mast Cells - enzymology, Mast Cells - physiology, Maternal & child health, Medicine, MicroRNA, MicroRNAs, MicroRNAs - metabolism, miRNA, Morpholines - pharmacology, NF-κB protein, Pediatrics, Phosphatidylinositol 3-Kinases - metabolism, Phosphorylation, Polymerase chain reaction, Protein Kinase Inhibitors - pharmacology, Proto-Oncogene Proteins c-akt - metabolism, Rats, Receptor, IGF Type 1 - metabolism, Ribonucleic acid, RNA, Rodents, Signal transduction, Signal Transduction - drug effects, Signaling, Smooth muscle, Stem cells, Target recognition, Transfection, Tuberculosis, Up-Regulation, Western blotting