PloS one, 2015-02, Vol.10 (2), p.e0117451-e0117451
2015
Details
- Autor(en) / Beteiligte
- Titel
- Probiotics (Lactobacillus rhamnosus R0011 and acidophilus R0052) reduce the expression of toll-like receptor 4 in mice with alcoholic liver disease
- Ist Teil von
- PloS one, 2015-02, Vol.10 (2), p.e0117451-e0117451
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The role of lipopolysaccharide (LPS) and toll-like receptor 4 (TLR 4) in the pathogenesis of alcoholic liver disease (ALD) has been widely established. We evaluated the biological effects of probiotics (Lactobacillus rhamnosus R0011 and acidophilus R0052), KRG (Korea red ginseng), and urushiol (Rhus verniciflua Stokes) on ALD, including their effects on normal and high-fat diet in mice. One hundred C57BL/6 mice were classified into normal (N) and high-fat diet (H) groups. Each group was divided into 5 sub-groups: control, alcohol, alcohol+probiotics, alcohol+KRG, and alcohol+urushiol. A liver function test, histology, electron-microscopy, interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6, and IL-10, and TLR 4 were evaluated and compared. In the N group, probiotics, KRG, and urushiol significantly reduced levels of TNF-α (12.3±5.1, 13.4±3.9, and 12.1±4.3 vs. 27.9±15.2 pg/mL) and IL-1β (108.4±39.4, 75.0±51.0, and 101.1±26.8 vs. 162.4±37.5 pg/mL), which were increased by alcohol. Alcohol-induced TLR 4 expression was reduced by probiotics and urushiol (0.7±0.2, and 0.8±0.1 vs. 1.0±0.3, p<0.001). In the H group, IL-10 was significantly increased by probiotics and KRG, compared with alcohol (25.3±15.6 and 20.4±6.2 vs. 7.6±5.6 pg/mL) and TLR 4 expression was reduced by probiotics (0.8±0.2 vs. 1.0±0.3, p = 0.007). Alcohol-induced TLR 4 expression was down-regulated by probiotics in the normal and high-fat diet groups. Probiotics, KRG, and urushiol might be effective in the treatment of ALD by regulating the gut-liver axis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0117451Titel-ID: cdi_plos_journals_1656050405
- Format
- –
- Schlagworte
- Alcohol use, Alcoholic beverages, Animals, Atherosclerosis, Bacteria, Biological effects, Biomarkers, Body Weight - drug effects, Catechols - pharmacology, Chromatography, Cytokines, Cytokines - metabolism, Diet, Diet, High-Fat - adverse effects, Ethanol, Fatty acids, Gene Expression Regulation - drug effects, Ginseng, High fat diet, Histology, House mouse, Immune system, Interleukin 10, Interleukin 6, Internal medicine, Laboratory animals, Lactobacillus acidophilus, Lactobacillus rhamnosus, Lipopolysaccharides, Liver, Liver - drug effects, Liver - metabolism, Liver - pathology, Liver - physiopathology, Liver cirrhosis, Liver diseases, Liver Diseases, Alcoholic - metabolism, Liver Diseases, Alcoholic - microbiology, Liver Diseases, Alcoholic - pathology, Liver Diseases, Alcoholic - physiopathology, Male, Medicine, Mice, Mice, Inbred C57BL, Microscopy, Panax - chemistry, Pathogenesis, Probiotics, Probiotics - pharmacology, Proteins, Rhus verniciflua, Rodents, Studies, TLR4 protein, Toll-Like Receptor 4 - metabolism, Toll-like receptors, Tumor necrosis factor, Tumor necrosis factor-TNF, Tumor necrosis factor-α