PloS one, 2014-11, Vol.9 (11), p.e113096-e113096
2014
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Details
- Autor(en) / Beteiligte
- Titel
- Upregulation of TPX2 by STAT3: identification of a novel STAT3 binding site
- Ist Teil von
- PloS one, 2014-11, Vol.9 (11), p.e113096-e113096
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- TPX2, a protein involved in mitosis, is considered a good marker for actively proliferating tissues, highly expressed in a number of cancer cells. We show the presence of high-affinity binding site for STAT3 in the 5'-flanking region of the Tpx2 gene, which is in vivo bound by activated STAT3. A specific STAT3 peptide inhibitor represses the expression of the Tpx2 gene and inhibits the binding of STAT3 to its consensus sequence in human cell lines where STAT3 is activated. These results indicate that activated STAT3 contributes to the over-expression of Tpx2 through the binding to an enhancer site.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0113096Titel-ID: cdi_plos_journals_1625576883
- Format
- –
- Schlagworte
- Apoptosis, Binding sites, Binding Sites - genetics, Biochemistry, Molecular Biology, Biology and life sciences, Blotting, Western, Cancer, Cell Cycle, Cell Cycle Proteins - genetics, Cell Cycle Proteins - metabolism, Cell Proliferation, Cells (Biology), Chemical properties, Chromatin Immunoprecipitation, Chromosomes, Conserved sequence, Cytokines, Deoxyribonucleic acid, DNA, Electrophoretic Mobility Shift Assay, Experiments, Fluorescent Antibody Technique, Gene expression, Gene Expression Regulation, Neoplastic, Genes, Genetic engineering, Growth factors, Humans, Kinases, Life Sciences, Medicine and Health Sciences, Melanoma, Microtubule-Associated Proteins - genetics, Microtubule-Associated Proteins - metabolism, Mitosis, Neoplasms - genetics, Neoplasms - metabolism, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Overexpression, Promoter Regions, Genetic, Protein binding, Proteins, Real-Time Polymerase Chain Reaction, Regulatory Sequences, Nucleic Acid, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger - genetics, Stat3 protein, STAT3 Transcription Factor - genetics, STAT3 Transcription Factor - metabolism, Thyroid gland, Tissues, Trans-Activators - metabolism, Transcription factors, Transcriptional Activation, Tumor Cells, Cultured, Tumors