Details

Autor(en) / Beteiligte

• Noulin, Florian
• Manesia, Javed Karim
• Rosanas-Urgell, Anna
• Erhart, Annette
• Borlon, Céline
• Van Den Abbeele, Jan
• d'Alessandro, Umberto
• Verfaillie, Catherine M
• Arez, Ana Paula

Titel

Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture

Ist Teil von

• PloS one, 2014-11, Vol.9 (11), p.e112496-e112496

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.