PloS one, 2014-10, Vol.9 (10), p.e109660
2014
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- Autor(en) / Beteiligte
- Titel
- Streptomyces coelicolor SCO4226 is a nickel binding protein
- Ist Teil von
- PloS one, 2014-10, Vol.9 (10), p.e109660
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The open reading frame SCO4226 of Streptomyces coelicolor A3(2) encodes an 82-residue hypothetical protein. Biochemical assays revealed that each SCO4226 dimer binds four nickel ions. To decipher the molecular function, we solved the crystal structures of SCO4226 in both apo- and nickel-bound (Ni-SCO4226) forms at 1.30 and 2.04 Å resolution, respectively. Each subunit of SCO4226 dimer adopts a canonical ferredoxin-like fold with five β-strands flanked by two α-helices. In the structure of Ni-SCO4226, four nickel ions are coordinated at the surface of the dimer. Further biochemical assays suggested that the binding of Ni2+ triggers the self-aggregation of SCO4226 in vitro. In addition, RT-qPCR assays demonstrated that the expression of SCO4226 gene in S. coelicolor is specifically up-regulated by the addition of Ni2+, but not other divalent ions such as Cu2+, Mn2+ or Co2+. All these results suggested that SCO4226 acts as a nickel binding protein, probably required for nickel sequestration and/or detoxification.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0109660Titel-ID: cdi_plos_journals_1604505721
- Format
- –
- Schlagworte
- Amino Acid Sequence, Assaying, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Bacteriology, BASIC BIOLOGICAL SCIENCES, Biology and Life Sciences, Carbon dioxide, Chemical properties, Cloning, Cobalt, Copper, Crystal structure, Crystallography, X-Ray, Crystals, Deoxyribonucleic acid, Detoxification, DNA, E coli, Escherichia coli, Ferredoxin, Gene expression, Gram-positive bacteria, Helices, Helicobacter pylori, Homeostasis, Ions, Laboratories, Life Sciences, Manganese ions, Microbiology and Parasitology, Models, Molecular, Molecular Sequence Data, Nickel, Nickel - metabolism, Nickel - pharmacology, Protein Binding, Protein Multimerization, Protein Structure, Quaternary, Proteins, Streptomyces, Streptomyces coelicolor, Structure, Transcription factors, Up-Regulation - drug effects