PloS one, 2014-09, Vol.9 (9), p.e106968-e106968
2014
Details
- Autor(en) / Beteiligte
- Titel
- Glutathione-triggered formation of a Fmoc-protected short peptide-based supramolecular hydrogel
- Ist Teil von
- PloS one, 2014-09, Vol.9 (9), p.e106968-e106968
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- A biocompatible method of glutathione (GSH) catalyzed disulfide bond reduction was used to form Fmoc-short peptide-based supramolecular hydrogels. The hydrogels could form in both buffer solution and cell culture medium containing 10% of Fetal Bovine Serum (FBS) within minutes. The hydrogel was characterized by rheology, transmission electron microscopy, and fluorescence emission spectra. Their potential in three dimensional (3D) cell culture was evaluated and the results indicated that the gel with a low concentration of the peptide (0.1 wt%) was suitable for 3D cell culture of 3T3 cells. This study provides an alternative candidate of supramolecular hydrogel for 3D cell culture and cell delivery.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0106968Titel-ID: cdi_plos_journals_1562148738
- Format
- –
- Schlagworte
- Amino Acids - chemistry, Animals, Biocompatibility, Biology, Biology and Life Sciences, Buffer solutions, Cell culture, Cell Culture Techniques, Chemical bonds, Collaboration, Cryoelectron Microscopy, Culture Media, Education, Electron microscopy, Emission analysis, Emission spectra, Engineering, Enzymes, Fluorenes - chemistry, Fluorescence, Glutathione, Glutathione - chemistry, Hydrogels, Hydrogels - chemical synthesis, Laboratories, Life sciences, Ligands, Mice, NIH 3T3 Cells, Peptides, Rheological properties, Rheology, Transmission electron microscopy