Details

Autor(en) / Beteiligte

• Kim, Jung-Yeon
• Goo, Youn-Kyoung
• Ji, So-Young
• Shin, Hyun-Il
• Han, Eun-Taek
• Hong, Yeonchul
• Chung, Dong-Il
• Cho, Shin-Hyung
• Lee, Won-Ja
• Tsuboi, Takafumi

Titel

Development and efficacy of real-time PCR in the diagnosis of vivax malaria using field samples in the Republic of Korea

Ist Teil von

• PloS one, 2014-08, Vol.9 (8), p.e105871-e105871

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The development of sensitive, rapid, and accurate diagnostic methods for vivax malaria is essential for the effective control of malaria in the Republic of Korea, where vivax malaria patients usually show low parasitemia. In this study, a TaqMan-based real-time polymerase chain reaction (PCR) method was established and compared with other PCR-based assays, including nested PCR, loop-mediated isothermal amplification, and multiplex PCR, using samples from febrile patients with suspected vivax malaria. The established real-time PCR had a high sensitivity (99.6%) and specificity (100%). Therefore, this sensitive, specific, rapid, and quantitative real-time PCR method could be used for the routine diagnosis of vivax malaria in the laboratory of the Korea National Institute of Health.