PloS one, 2014-08, Vol.9 (8), p.e104209
2014
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- Autor(en) / Beteiligte
- Titel
- Oxygen-carbon nanotubes as a chemotherapy sensitizer for paclitaxel in breast cancer treatment
- Ist Teil von
- PloS one, 2014-08, Vol.9 (8), p.e104209
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To study the in vivo and in vitro effects of adding oxygen carbon nanotubes (CNTs) to chemotherapy for breast cancer. MCF-7 and SK-BR-3 breast cancer cells were co-cultured with paclitaxel and then exposed to oxygen-CNTs under hypoxic conditions. Cell proliferation, viability, and apoptosis rate were analyzed. Hypoxia-inducible factor-1 alpha (HIF-1α) expression was measured using reverse transcription-polymerase chain reaction (RT-PCR) and western blot. Nude mice were used as a human breast cancer model to explore the impact of oxygen-CNTs on the in vivo chemotherapeutic effect of paclitaxel. Oxygen-CNTs had no significant effects on the growth of breast cancer cells under normoxia and hypoxia. However, in the hypoxic environment, oxygen-CNTs significantly enhanced the inhibitory effect of paclitaxel on cell proliferation, as well as the apoptosis rate. Under hypoxia, downregulation of HIF-1α and upregulation of caspase-3, caspase-8, caspase-9, LC3 and Beclin-1 were observed when paclitaxel was combined with oxygen-CNT. Furthermore, addition of oxygen-CNTs to chemotherapy was found to significantly reduce tumor weight in the tumor-bearing mice model. Oxygen-CNTs can significantly increase the chemotherapeutic effect of paclitaxel on breast cancer cells. Oxygen-CNTs may be a potential chemosensitizer in breast cancer therapy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0104209Titel-ID: cdi_plos_journals_1551148419
- Format
- –
- Schlagworte
- Animals, Apoptosis, Apoptosis - drug effects, Biology and Life Sciences, Breast cancer, Breast Neoplasms - drug therapy, Breast Neoplasms - genetics, Breast Neoplasms - pathology, Cancer, Cancer therapies, Carbon, Carbon nanotubes, Caspase, Caspase-3, Caspase-8, Caspase-9, Cell growth, Cell Hypoxia - drug effects, Cell proliferation, Cell Proliferation - drug effects, Chemotherapy, Drug Resistance, Neoplasm - drug effects, Drug therapy, Engineering and Technology, Female, Gene expression, Humans, Hypoxia, Hypoxia-inducible factor 1, Hypoxia-inducible factor 1a, Hypoxia-inducible factors, In vivo methods and tests, Laboratory animals, MCF-7 Cells, Medical research, Medicine, Medicine and Health Sciences, Mice, Nanotechnology, Nanotubes, Nanotubes, Carbon - chemistry, Oncology, Oxygen, Oxygen - administration & dosage, Oxygen - chemistry, Paclitaxel, Paclitaxel - administration & dosage, Penicillin, Physical Sciences, Polymerase chain reaction, Radiation therapy, Research and Analysis Methods, Reverse transcription, Surgery, Tumors, Viability, Weight reduction, Xenograft Model Antitumor Assays