PloS one, 2014-07, Vol.9 (7), p.e102849-e102849
2014
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Details
- Autor(en) / Beteiligte
- Titel
- Vanadate influence on metabolism of sugar phosphates in fungus Phycomyces blakesleeanus
- Ist Teil von
- PloS one, 2014-07, Vol.9 (7), p.e102849-e102849
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in 31P NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (31P NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0102849Titel-ID: cdi_plos_journals_1545995245
- Format
- –
- Schlagworte
- Adenosine Triphosphate - metabolism, Biology and Life Sciences, Biophysics, Carbohydrate metabolism, Carbohydrate Metabolism - physiology, Catalysis, Dehydrogenases, Enzymes, Experiments, Fructose, Fungi, Fungi - metabolism, Genomes, Glucosamine, Glucose, Glucose metabolism, Glycogen, Glycogen phosphorylase, Glycogen synthesis, Glycolysis, Glycolysis - physiology, High performance liquid chromatography, Kinases, Kinetics, Life sciences, Liquid chromatography, Magnetic Resonance Spectroscopy - methods, Metabolism, Mycelia, NMR, Nuclear magnetic resonance, Phosphatase, Phosphate, Phosphate esters, Phosphates, Phosphofructokinase, Phosphoglucomutase, Phosphorylase, Phycomyces - metabolism, Physical Sciences, Physiological aspects, Physiology, Polyphosphates, Polyphosphates - metabolism, Research and analysis methods, Saccharomyces cerevisiae, Sugar, Sugar Phosphates - metabolism, Uridine Diphosphate Glucose - metabolism, Vanadate, Vanadates - metabolism, Vanadium, Yeast