PloS one, 2014-06, Vol.9 (6), p.e100268
2014
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Details
- Autor(en) / Beteiligte
- Titel
- The serine-threonine protein kinase PAK4 is dispensable in zebrafish: identification of a morpholino-generated pseudophenotype
- Ist Teil von
- PloS one, 2014-06, Vol.9 (6), p.e100268
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- TALEN-based inactivation of the zebrafish pak4 gene resulted in embryos and adult fish that appear normal and fertile. This is in contrast to our previously published studies which were based on the use of antisense morpholino oligonucleotides (MOs). We have excluded potential explanations such as gene duplication, alternate splicing, cryptic initiation of translation, and translation-independent RNA function. Our conclusion is that pak4 is dispensable in zebrafish, and that even when corroborated by robust controls, such as RNA rescue, MOs may elicit misleading pseudophenotypes that do not correspond to results obtained by genetic mutations, and should thus be used with caution.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0100268Titel-ID: cdi_plos_journals_1537650825
- Format
- –
- Schlagworte
- Alleles, Alternative splicing, Animals, Antisense oligonucleotides, Apoptosis, Base Sequence, Biology and Life Sciences, Childrens health, Danio rerio, Deactivation, Dioxins, Embryos, Endonucleases - metabolism, Female, Gene duplication, Gene expression, Gene Knockout Techniques, Genomics, Genotype & phenotype, Hydrocarbons, Inactivation, INDEL Mutation - genetics, Kinases, Male, Molecular Sequence Data, Morpholinos - pharmacology, Mutation, Mutation - genetics, Oligonucleotides, p21-Activated Kinases - genetics, p21-Activated Kinases - metabolism, Phenotype, Protein kinase, Proteins, Ribonucleic acid, RNA, RNA - genetics, RNA - metabolism, Robust control, Signal transduction, Stress, Physiological - drug effects, Stress, Physiological - genetics, Threonine, Trans-Activators - metabolism, Translation, Translation initiation, Tumor necrosis factor-TNF, Zebrafish, Zebrafish - embryology, Zebrafish - metabolism, Zebrafish Proteins - genetics, Zebrafish Proteins - metabolism