Details

Autor(en) / Beteiligte

• Ali, Hazrat
• Ries, Marco I
• Lankhorst, Peter P
• van der Hoeven, Rob A M
• Schouten, Olaf L
• Noga, Marek
• Hankemeier, Thomas
• van Peij, Noël N M E
• Bovenberg, Roel A L
• Vreeken, Rob J
• Driessen, Arnold J M
• Andersen, Mikael Rørdam

Titel

A non-canonical NRPS is involved in the synthesis of fungisporin and related hydrophobic cyclic tetrapeptides in Penicillium chrysogenum

Ist Teil von

• PloS one, 2014-06, Vol.9 (6), p.e98212

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The filamentous fungus Penicillium chrysogenum harbors an astonishing variety of nonribosomal peptide synthetase genes, which encode proteins known to produce complex bioactive metabolites from simple building blocks. Here we report a novel non-canonical tetra-modular nonribosomal peptide synthetase (NRPS) with microheterogenicity of all involved adenylation domains towards their respective substrates. By deleting the putative gene in combination with comparative metabolite profiling various unique cyclic and derived linear tetrapeptides were identified which were associated with this NRPS, including fungisporin. In combination with substrate predictions for each module, we propose a mechanism for a 'trans-acting' adenylation domain.