Details

Autor(en) / Beteiligte

• Shearer, Alexander G
• Altman, Tomer
• Rhee, Christine D
• Lespinet, Olivier

Titel

Finding sequences for over 270 orphan enzymes

Ist Teil von

• PloS one, 2014-05, Vol.9 (5), p.e97250-e97250

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Despite advances in sequencing technology, there are still significant numbers of well-characterized enzymatic activities for which there are no known associated sequences. These 'orphan enzymes' represent glaring holes in our biological understanding, and it is a top priority to reunite them with their coding sequences. Here we report a methodology for resolving orphan enzymes through a combination of database search and literature review. Using this method we were able to reconnect over 270 orphan enzymes with their corresponding sequence. This success points toward how we can systematically eliminate the remaining orphan enzymes and prevent the introduction of future orphan enzymes.