PloS one, 2014-05, Vol.9 (5), p.e96252-e96252
2014
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Details
- Autor(en) / Beteiligte
- Titel
- Dexamethasone palmitate ameliorates macrophages-rich graft-versus-host disease by inhibiting macrophage functions
- Ist Teil von
- PloS one, 2014-05, Vol.9 (5), p.e96252-e96252
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Macrophage infiltration of skin GVHD lesions correlates directly with disease severity, but the mechanisms underlying this relationship remain unclear and GVHD with many macrophages is a therapeutic challenge. Here, we characterize the macrophages involved in GVHD and report that dexamethasone palmitate (DP), a liposteroid, can ameliorate such GVHD by inhibiting macrophage functions. We found that host-derived macrophages could exacerbate GVHD in a mouse model through expression of higher levels of pro-inflammatory TNF-α and IFN-γ, and lower levels of anti-inflammatory IL-10 than resident macrophages in mice without GVHD. DP significantly decreased the viability and migration capacity of primary mouse macrophages compared to conventional dexamethasone in vitro. DP treatment on day 7 and day 14 decreased macrophage number, and attenuated GVHD score and subsequent mortality in a murine model. This is the first study to provide evidence that therapy for GVHD should be changed on the basis of infiltrating cell type.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0096252Titel-ID: cdi_plos_journals_1522141057
- Format
- –
- Schlagworte
- Animal models, Animals, Antigens, Biology and Life Sciences, Bone marrow, Cell adhesion & migration, Cell Line, Central nervous system depressants, Comparative analysis, Cytoplasm, Dendritic cells, Dexamethasone, Dexamethasone - therapeutic use, Disease Models, Animal, Female, Graft versus host disease, Graft vs Host Disease - drug therapy, Graft vs Host Disease - immunology, Graft-versus-host reaction, Health aspects, Hematology, Hospitals, Humans, Immunohistochemistry, Infiltration, Inflammation, Interferon, Interleukin 10, Laboratory animals, Lesions, Lymphocytes - drug effects, Lymphocytes - immunology, Macrophages, Macrophages - drug effects, Macrophages - immunology, Male, Mathematical models, Medicine, Medicine and Health Sciences, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Oncology, Palmitic acid, Research and Analysis Methods, Skin, Skin diseases, Steroids, Steroids (Organic compounds), Transplants & implants, Tumor necrosis factor-α, University graduates, Viability, γ-Interferon