Details

Autor(en) / Beteiligte

• Liachko, Ivan
• Youngblood, Rachel A
• Tsui, Kyle
• Bubb, Kerry L
• Queitsch, Christine
• Raghuraman, M K
• Nislow, Corey
• Brewer, Bonita J
• Dunham, Maitreya J
• Newlon, Carol S.

Titel

GC-rich DNA elements enable replication origin activity in the methylotrophic yeast Pichia pastoris

Ist Teil von

• PLoS genetics, 2014-03, Vol.10 (3), p.e1004169-e1004169

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The well-studied DNA replication origins of the model budding and fission yeasts are A/T-rich elements. However, unlike their yeast counterparts, both plant and metazoan origins are G/C-rich and are associated with transcription start sites. Here we show that an industrially important methylotrophic budding yeast, Pichia pastoris, simultaneously employs at least two types of replication origins--a G/C-rich type associated with transcription start sites and an A/T-rich type more reminiscent of typical budding and fission yeast origins. We used a suite of massively parallel sequencing tools to map and dissect P. pastoris origins comprehensively, to measure their replication dynamics, and to assay the global positioning of nucleosomes across the genome. Our results suggest that some functional overlap exists between promoter sequences and G/C-rich replication origins in P. pastoris and imply an evolutionary bifurcation of the modes of replication initiation.