PloS one, 2014-04, Vol.9 (4), p.e94536-e94536
2014
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Details
- Autor(en) / Beteiligte
- Titel
- Decreased core-fucosylation contributes to malignancy in gastric cancer
- Ist Teil von
- PloS one, 2014-04, Vol.9 (4), p.e94536-e94536
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- The object of the study is to identify N-glycan profiling changes associated with gastric cancer and explore the impact of core-fucosylation on biological behaviors of human gastric cancer cells. A total of 244 subjects including gastric cancer, gastric ulcer and healthy control were recruited. N-glycan profiling from serum and total proteins in gastric tissues was analyzed by DNA sequencer-assisted fluorophore-assisted capillary electrophoresis. The abundance of total core-fucosylated residues and the expression of enzymes involved in core-fucosylation were analyzed with lectin blot, quantitative reverse transcription-polymerase chain reaction, western blot, Immunohistochemical staining and lectin-histochemical staining. The recombinant plasmids of GDP-fucose transporter and α-1,6-fucosyltransferase (Fut8) were constructed and transfected into gastric cancer cell lines BGC-823 and SGC-7901. CCK-8 and wound healing assay were used to assess the functional impact of core-fucosylation modulation on cell proliferation and migration. Characteristic serum N-glycan profiles were found in gastric cancer. Compared with the healthy control, a trianntenary structure abundance, peak 9 (NA3Fb), was increased significantly in gastric cancer, while the total abundance of core-fucosylated residues (sumfuc) was decreased. Core-fucosylated structures, peak6(NA2F) and peak7(NA2FB) were deceased in gastric tumor tissues when compared with that in adjacent non-tumor tissues. Consistently, lens culinaris agglutinin (LCA)-binding proteins were decreased significantly in sera of gastric cancer, and protein level of Fut8 was decreased significantly in gastric tumor tissues compared with that in adjacent non-tumor tissues. Upregulation of GDP-Tr and Fut8 could inhibit proliferation, but had no significant influence on migration of BGC-823 and SGC-7901 cells. Core-fucosylation is down regulated in gastric cancer. Upregulation of core-fucosylation could inhibit proliferation of the human gastric cancer cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0094536Titel-ID: cdi_plos_journals_1516461550
- Format
- –
- Schlagworte
- Abundance, alpha-L-Fucosidase - metabolism, Analysis, Biology and Life Sciences, Biomarkers, Tumor - metabolism, Cancer, Cancer cells, Capillary electrophoresis, Care and treatment, Case-Control Studies, Cell Line, Tumor, Cell migration, Cell Movement, Cell Proliferation, Cholecystokinin, Demography, Deoxyribonucleic acid, Diagnosis, Disease Progression, DNA, Female, Fucose, Fucose - metabolism, Fucosyltransferases - metabolism, Gastric cancer, GDP-fucose, Glycan, Glycomics, Glycoproteins, Hospitals, Human behavior, Humans, Immunoglobulins, Immunohistochemistry, Laboratories, Male, Malignancy, Medicine, Medicine and Health Sciences, Middle Aged, N-Acetylneuraminic Acid - chemistry, N-Acetylneuraminic Acid - metabolism, Nucleotide sequence, Ovarian cancer, Plasmids, Polymerase chain reaction, Polysaccharides - blood, Polysaccharides - chemistry, Prostate, Proteins, Proteomics, Residues, Reverse transcription, Risk factors, Signal transduction, Staining, Stomach cancer, Stomach Neoplasms - blood, Stomach Neoplasms - enzymology, Stomach Neoplasms - metabolism, Stomach Neoplasms - pathology, Stomach Ulcer - metabolism, Stomach Ulcer - pathology, Surgery, Tissues, Tumor cell lines, Tumors, Ulcers, Wound Healing