PloS one, 2014-03, Vol.9 (3), p.e92747-e92747
2014
Details
- Autor(en) / Beteiligte
- Titel
- HIV-2 integrase polymorphisms and longitudinal genotypic analysis of HIV-2 infected patients failing a raltegravir-containing regimen
- Ist Teil von
- PloS one, 2014-03, Vol.9 (3), p.e92747-e92747
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- To characterize the HIV-2 integrase gene polymorphisms and the pathways to resistance of HIV-2 patients failing a raltegravir-containing regimen, we studied 63 integrase strand transfer inhibitors (INSTI)-naïve patients, and 10 heavily pretreated patients exhibiting virological failure while receiving a salvage raltegravir-containing regimen. All patients were infected by HIV-2 group A. 61.4% of the integrase residues were conserved, including the catalytic motif residues. No INSTI-major resistance mutations were detected in the virus population from naïve patients, but two amino acids that are secondary resistance mutations to INSTIs in HIV-1 were observed. The 10 raltegravir-experienced patients exhibited resistance mutations via three main genetic pathways: N155H, Q148R, and eventually E92Q - T97A. The 155 pathway was preferentially used (7/10 patients). Other mutations associated to raltegravir resistance in HIV-1 were also observed in our HIV-2 population (V151I and D232N), along with several novel mutations previously unreported. Data retrieved from this study should help build a more robust HIV-2-specific algorithm for the genotypic interpretation of raltegravir resistance, and contribute to improve the clinical monitoring of HIV-2-infected patients.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0092747Titel-ID: cdi_plos_journals_1511110011
- Format
- –
- Schlagworte
- Acid resistance, Acquired immune deficiency syndrome, AIDS, Amino acids, Analysis, Antiretroviral agents, Antiretroviral drugs, Antiviral agents, Biology and Life Sciences, Catalysis, Disease resistance, Drug Resistance, Viral - genetics, Genetic aspects, Genetic polymorphisms, Genotype, HIV, HIV (Viruses), HIV Infections - drug therapy, HIV Integrase - genetics, HIV Integrase Inhibitors - therapeutic use, HIV-1 - drug effects, HIV-2 - drug effects, HIV-2 - genetics, Human immunodeficiency virus, Humans, Integrase, Medical research, Medicine and health sciences, Medicine, Experimental, Mutation, Patients, Polymorphism, Genetic - genetics, Proteins, Pyrrolidinones - therapeutic use, Raltegravir Potassium, Residues, Salvage, Viruses