PloS one, 2014-03, Vol.9 (3), p.e90807
2014
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- Autor(en) / Beteiligte
- Titel
- Chaihuang-Yishen granule inhibits diabetic kidney disease in rats through blocking TGF-β/Smad3 signaling
- Ist Teil von
- PloS one, 2014-03, Vol.9 (3), p.e90807
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Increasing evidence shows that TGF-β1 is a key mediator in diabetic nephropathy (DN) and induces renal fibrosis positively by Smad3 but negatively by Smad7. However, treatment of DN by blocking the TGF-β/Smad pathway remains limited. The present study investigated the anti-fibrotic effect of a traditional Chinese medicine, Chaihuang-Yishen granule (CHYS), on DN. Protective role of CHYS in DN was examined in an accelerated type 1 DN induced by streptozotocin in uninephrectomized Wistar rats. CHYS, at a dose of 0.56 g/kg body weight, was administered by a daily gastric gavage for 20 weeks and the therapeutic effect and potential mechanisms of CHYS on diabetic kidney injury were examined. Treatment with CHYS attenuated diabetic kidney injury by significantly inhibiting 24-h proteinuria and progressive renal fibrosis including glomerulosclerotic index, tubulointerstitial fibrosis index, and upregulation of extracellular matrix (collagen I, IV, and fibronectin), despite the same levels of blood glucose. Further studies revealed that inhibition of renal fibrosis in CHYS-treated diabetic rats was associated with inhibition of TGF-β1/Smad3 signaling as demonstrated by upregulation of Smad7 but downregulation of TGF-β1, TGF-β receptors, activation of Smad3, and expression of miRNA-21. CHYS may be a therapeutic agent for DN. CHYS attenuates DN by blocking TGF-β/Smad3-mediated renal fibrosis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0090807Titel-ID: cdi_plos_journals_1508765065
- Format
- –
- Schlagworte
- Acids, Animals, Blood Glucose - metabolism, Body weight, Chemical compounds, Chinese medicine, Collagen (type I), Diabetes, Diabetes mellitus, Diabetes Mellitus, Experimental - blood, Diabetes Mellitus, Experimental - genetics, Diabetes Mellitus, Experimental - pathology, Diabetic Nephropathies - drug therapy, Diabetic Nephropathies - genetics, Diabetic Nephropathies - metabolism, Diabetic Nephropathies - pathology, Diabetic nephropathy, Drug dosages, Drugs, Chinese Herbal - pharmacology, Extracellular matrix, Fibronectin, Fibrosis, Gene Expression Regulation, Granular materials, Growth factors, Health sciences, Hospitals, Hypoglycemic Agents - pharmacology, Inhibition, Kidney diseases, Male, Medicine and Health Sciences, MicroRNAs, miRNA, Nephropathy, Pharmacology, Proteinuria, Rats, Rats, Wistar, Receptors, Receptors, Transforming Growth Factor beta - antagonists & inhibitors, Receptors, Transforming Growth Factor beta - genetics, Receptors, Transforming Growth Factor beta - metabolism, Research design, Rodents, Signal Transduction - drug effects, Signaling, Smad protein, Smad3 protein, Smad3 Protein - antagonists & inhibitors, Smad3 Protein - genetics, Smad3 Protein - metabolism, Smad7 protein, Smad7 Protein - agonists, Smad7 Protein - genetics, Smad7 Protein - metabolism, Solvents, Streptozocin, Traditional Chinese medicine, Transforming Growth Factor beta1 - antagonists & inhibitors, Transforming Growth Factor beta1 - genetics, Transforming Growth Factor beta1 - metabolism, Transforming growth factor-b1