PloS one, 2014-02, Vol.9 (2), p.e80199
2014
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- Autor(en) / Beteiligte
- Titel
- Wound healing activity and mechanisms of action of an antibacterial protein from the venom of the eastern diamondback rattlesnake (Crotalus adamanteus)
- Ist Teil von
- PloS one, 2014-02, Vol.9 (2), p.e80199
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Basic phospholipase A2 was identified from the venom of the eastern diamondback rattlesnake. The Crotalus adamanteus toxin-II (CaTx-II) induced bactericidal effects (7.8 µg/ml) on Staphylococcus aureus, while on Burkholderia pseudomallei (KHW), and Enterobacter aerogenes were killed at 15.6 µg/ml. CaTx-II caused pore formation and membrane damaging effects on the bacterial cell wall. CaTx-II was not cytotoxic on lung (MRC-5), skin fibroblast (HEPK) cells and in mice. CaTx-II-treated mice showed significant wound closure and complete healing by 16 days as compared to untreated controls (**P<0.01). Histological examination revealed enhanced collagen synthesis and neovascularization after treatment with CaTx-II versus 2% Fusidic Acid ointment (FAO) treated controls. Measurement of tissue cytokines revealed that interleukin-1 beta (IL-1β) expression in CaTx-II treated mice was significantly suppressed versus untreated controls. In contrast, cytokines involved in wound healing and cell migration i.e., monocyte chemotactic protein-1 (MCP-1), fibroblast growth factor-basic (FGF-b), chemokine (KC), granulocyte-macrophage colony-stimulating factor (GM-CSF) were significantly enhanced in CaTx-II treated mice, but not in the controls. CaTx-II also modulated nuclear factor-kappa B (NF-κB) activation during skin wound healing. The CaTx-II protein highlights distinct snake proteins as a potential source of novel antimicrobial agents with significant therapeutic application for bacterial skin infections.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0080199Titel-ID: cdi_plos_journals_1498237498
- Format
- –
- Schlagworte
- Aerogenes, Angiogenesis, Animals, Anti-Bacterial Agents - chemistry, Anti-Bacterial Agents - isolation & purification, Anti-Bacterial Agents - pharmacology, Anti-Bacterial Agents - toxicity, Antibacterial agents, Antibiotics, Antimicrobial agents, Bacteria, Bacteria - drug effects, Bacteria - growth & development, Bacteria - ultrastructure, Bacterial infections, Biology, Burkholderia, Casualties, Cell Death - drug effects, Cell Line, Cell Membrane - drug effects, Cell Membrane - metabolism, Cell migration, Cell Survival - drug effects, Cell walls, Chemical compounds, Chromatography, Collagen, Collagen - metabolism, Colony Count, Microbial, Colony-stimulating factor, Crotalid Venoms - chemistry, Crotalid Venoms - isolation & purification, Crotalid Venoms - pharmacology, Crotalid Venoms - toxicity, Crotalinae, Crotalus - metabolism, Crotalus adamanteus, Crotalus durissus terrificus, Cytokines, Cytokines - metabolism, Cytotoxicity, Epithelium - drug effects, Epithelium - pathology, Fibroblast growth factor, Fibroblast growth factors, Fusidic acid, Granulocyte-macrophage colony-stimulating factor, Humans, Infectious diseases, Inflammation Mediators - metabolism, Interleukin, Interleukin 1, L-Lactate Dehydrogenase - metabolism, Legal medicine, Lungs, Macrophages, Male, Medicine, Mice, Microbial Sensitivity Tests, Monocyte chemoattractant protein 1, Neovascularization, NF-kappa B - metabolism, NF-κB protein, Ointments, Peptides, Pharmacology, Phospholipase, Phospholipase A2, Pore formation, Proteins, Rattlesnakes, Rodents, Sequence Analysis, Protein, Skin, Skin - drug effects, Skin - pathology, Snakes, Staphylococcus aureus, Staphylococcus aureus infections, Vascular endothelial growth factor, Vascularization, Venom, Venoms, Viperidae, Wound healing, Wound Healing - drug effects