PloS one, 2014-02, Vol.9 (2), p.e87329
2014
Details
- Autor(en) / Beteiligte
- Titel
- Non-replicating Mycobacterium tuberculosis elicits a reduced infectivity profile with corresponding modifications to the cell wall and extracellular matrix
- Ist Teil von
- PloS one, 2014-02, Vol.9 (2), p.e87329
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- A key feature of Mycobacterium tuberculosis is its ability to become dormant in the host. Little is known of the mechanisms by which these bacilli are able to persist in this state. Therefore, the focus of this study was to emulate environmental conditions encountered by M. tuberculosis in the granuloma, and determine the effect of such conditions on the physiology and infectivity of the organism. Non-replicating persistent (NRP) M. tuberculosis was established by the gradual depletion of nutrients in an oxygen-replete and controlled environment. In contrast to rapidly dividing bacilli, NRP bacteria exhibited a distinct phenotype by accumulating an extracellular matrix rich in free mycolate and lipoglycans, with increased arabinosylation. Microarray studies demonstrated a substantial down-regulation of genes involved in energy metabolism in NRP bacteria. Despite this reduction in metabolic activity, cells were still able to infect guinea pigs, but with a delay in the development of disease when compared to exponential phase bacilli. Using these approaches to investigate the interplay between the changing environment of the host and altered physiology of NRP bacteria, this study sheds new light on the conditions that are pertinent to M. tuberculosis dormancy and how this organism could be establishing latent disease.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0087329Titel-ID: cdi_plos_journals_1495651522
- Format
- –
- Schlagworte
- Adaptation, Adaptation, Physiological - drug effects, Adaptation, Physiological - genetics, Animals, Bacilli, Bacteria, Bacterial Load - drug effects, Bacterial Load - genetics, Besra, Biology, Carbohydrates, Carbohydrates - chemistry, Carbon, Carbon - pharmacology, Cell Wall - drug effects, Cell Wall - metabolism, Cell walls, Chemistry, Chromatography, Thin Layer, Development and progression, Dormancy, Electrophoresis, Polyacrylamide Gel, Energy metabolism, Environmental conditions, Extracellular matrix, Extracellular Matrix - drug effects, Extracellular Matrix - metabolism, Fatty acids, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Bacterial - drug effects, Gene regulation, Granuloma, Growth models, Guinea Pigs, Hypoxia, Infections, Infectivity, Kinases, Lipids, Mathematics, Medicine, Metabolism, Mice, Molecular Sequence Annotation, Multigene Family, Mycobacterium Infections - genetics, Mycobacterium Infections - microbiology, Mycobacterium Infections - pathology, Mycobacterium tuberculosis, Mycobacterium tuberculosis - genetics, Mycobacterium tuberculosis - growth & development, Mycobacterium tuberculosis - pathogenicity, Mycobacterium tuberculosis - ultrastructure, Nutrients, Oxygen, Physiological aspects, Physiology, Polysorbates - pharmacology, Proteins, Public health, Replicating, Replication, Studies, Tuberculosis, Up-Regulation - genetics