Details
- Autor(en) / Beteiligte
- Titel
- Adrenergic β2 receptor activation stimulates anti-inflammatory properties of dendritic cells in vitro
- Ist Teil von
- PloS one, 2014, Vol.9 (1), p.e85086
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Vagal nerve efferent activation has been shown to ameliorate the course of many inflammatory disease states. This neuro-modulatory effect has been suggested to rest on acetylcholine receptor (AChR) activation on tissue macrophages or dendritic cells (DCs). In more recent studies, vagal anti-inflammatory activity was shown involve adrenergic, splenic, pathways. Here we provide evidence that the adrenergic, rather than cholinergic, receptor activation on bone marrow derived DCs results in enhanced endocytosis uptake, enhanced IL-10 production but a decreased IL-6, IL-12p70 and IL-23 production. In antigen specific T cell stimulation assays, adrenergic β2 receptor activation on bone marrow DCs led to an enhanced potential to induce Foxp3 positive suppressive Treg cells. These effects were independent of IL10-R activation, TGFβ release, or retinoic acid (RA) secretion. Hence, adrenergic receptor β2 activation modulates DC function resulting in skewing towards anti-inflammatory T cell phenotypes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0085086Titel-ID: cdi_plos_journals_1491120289
- Format
- –
- Schlagworte
- Acetylcholine receptors, Adrenergic beta-Agonists - pharmacology, Adrenergic receptors, Albuterol - pharmacology, Animals, Anti-inflammatory agents, Antigens, Biology, Bone marrow, Bone Marrow Cells - drug effects, Bone Marrow Cells - metabolism, Brain research, Cell activation, Cells, Cultured, Cloning, Cytokines, Cytokines - metabolism, Dendritic cells, Dendritic Cells - drug effects, Dendritic Cells - metabolism, Dextrans - pharmacokinetics, Disease, Endocytosis, Endocytosis - drug effects, Epinephrine - pharmacology, Experiments, Female, Flow Cytometry, Fluorescein-5-isothiocyanate - analogs & derivatives, Fluorescein-5-isothiocyanate - pharmacokinetics, Forkhead Transcription Factors - metabolism, Foxp3 protein, Growth factors, Homeostasis, Immune system, Immunology, Inflammation, Inflammation Mediators - metabolism, Interleukin 1, Interleukin 10, Interleukin 12, Interleukin 23, Interleukin 6, Interleukin-10 - metabolism, Interleukin-12 - metabolism, Interleukin-23 - metabolism, Interleukin-6 - metabolism, Laboratories, Liver, Lymphocytes, Lymphocytes T, Macrophages, Medicine, Mice, Mice, Inbred C57BL, Nervous system, Neuropeptides, Receptor mechanisms, Receptors, Adrenergic, beta-2 - genetics, Receptors, Adrenergic, beta-2 - metabolism, Retinoic acid, Reverse Transcriptase Polymerase Chain Reaction, Rodents, Spleen, T-Lymphocytes, Regulatory - drug effects, T-Lymphocytes, Regulatory - metabolism, Vagus nerve