PloS one, 2014-01, Vol.9 (1), p.e86173-e86173
2014
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- Autor(en) / Beteiligte
- Titel
- Targeting cancer cells with reactive oxygen and nitrogen species generated by atmospheric-pressure air plasma
- Ist Teil von
- PloS one, 2014-01, Vol.9 (1), p.e86173-e86173
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The plasma jet has been proposed as a novel therapeutic method for cancer. Anticancer activity of plasma has been reported to involve mitochondrial dysfunction. However, what constituents generated by plasma is linked to this anticancer process and its mechanism of action remain unclear. Here, we report that the therapeutic effects of air plasma result from generation of reactive oxygen/nitrogen species (ROS/RNS) including H2O2, Ox, OH-, •O2, NOx, leading to depolarization of mitochondrial membrane potential and mitochondrial ROS accumulation. Simultaneously, ROS/RNS activate c-Jun NH2-terminal kinase (JNK) and p38 kinase. As a consequence, treatment with air plasma jets induces apoptotic death in human cervical cancer HeLa cells. Pretreatment of the cells with antioxidants, JNK and p38 inhibitors, or JNK and p38 siRNA abrogates the depolarization of mitochondrial membrane potential and impairs the air plasma-induced apoptotic cell death, suggesting that the ROS/RNS generated by plasma trigger signaling pathways involving JNK and p38 and promote mitochondrial perturbation, leading to apoptosis. Therefore, administration of air plasma may be a feasible strategy to eliminate cancer cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0086173Titel-ID: cdi_plos_journals_1490992783
- Format
- –
- Schlagworte
- Air, Air plasma, Anticancer properties, Antioxidants, Antioxidants (Nutrients), Antioxidants - pharmacology, Antitumor activity, Apoptosis, Apoptosis - drug effects, Atmospheric Pressure, Biology, Cancer, Cancer cells, Cancer therapies, Cancer treatment, Cell death, Cervical cancer, Cervix, Computer engineering, Depolarization, Enzyme Activation - drug effects, Extracellular Space - metabolism, Health aspects, HeLa Cells, Humans, Hydrogen peroxide, Intracellular Space - metabolism, Ionizing radiation, JNK Mitogen-Activated Protein Kinases - metabolism, JNK protein, Kinases, Life sciences, MAP Kinase Signaling System - drug effects, Membrane potential, Membrane Potential, Mitochondrial - drug effects, Mitochondria, Mitochondria - drug effects, Mitochondria - metabolism, Neoplasms - metabolism, Neoplasms - pathology, Nitrogen, Nitrogen oxides, Oxidative stress, Oxygen, p38 Mitogen-Activated Protein Kinases - metabolism, Phosphorylation - drug effects, Plasma, Plasma Gases - chemistry, Plasma jets, Plasma physics, Proteins, Reactive nitrogen species, Reactive Nitrogen Species - metabolism, Reactive oxygen species, Reactive Oxygen Species - metabolism, Rodents, Signal transduction, Signaling, siRNA, Spectrum Analysis, Transcription factors, Tumor necrosis factor-TNF