PloS one, 2014-01, Vol.9 (1), p.e84434-e84434
2014
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- Autor(en) / Beteiligte
- Titel
- Role of PON in anoxia-reoxygenation injury: a Drosophila melanogaster transgenic model
- Ist Teil von
- PloS one, 2014-01, Vol.9 (1), p.e84434-e84434
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Paraoxonase 1 (PON1) is a protein found associated with high density lipoprotein (HDL), thought to prevent oxidative modification of low-density lipoprotein (LDL). This enzyme has been implicated in lowering the risk of cardiovascular disease. Anoxia-reoxygenation and oxidative stress are important elements in cardiovascular and cerebrovascular disease. However, the role of PON1 in anoxia-reoxygenation or anoxic injury is unclear. We hypothesize that PON1 prevents anoxia-reoxygenation injury. We set out to determine whether PON1 expression in Drosophila melanogaster protects against anoxia-reoxygenation (A-R) induced injury. Wild type (WT) and transgenic PON1 flies were exposed to anoxia (100% Nitrogen) for different time intervals (from 1 to 24 hours). After the anoxic period, flies were placed in room air for reoxygenation. Activity and survival of flies was then recorded. Within 5 minutes of anoxia, all flies fell into a stupor state. After reoxygenation, survivor flies resumed activity with some delay. Interestingly, transgenic flies recovered from stupor later than WT. PON1 transgenic flies had a significant survival advantage after A-R stress compared with WT. The protection conferred by PON1 expression was present regardless of the age or dietary restriction. Furthermore, PON1 expression exclusively in CNS conferred protection. Our results support the hypothesis that PON1 has a protective role in anoxia-reoxygenation injury, and its expression in the CNS is sufficient and necessary to provide a 100% survival protection.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0084434Titel-ID: cdi_plos_journals_1474849985
- Format
- –
- Schlagworte
- Animals, Animals, Genetically Modified, Anoxia, Aryldialkylphosphatase - genetics, Aryldialkylphosphatase - metabolism, Atherosclerosis, Biology, Cardiovascular disease, Cardiovascular diseases, Central nervous system, Central Nervous System - metabolism, Coronary vessels, Diabetes, Diet, Dietary restrictions, Disease Models, Animal, Drosophila, Drosophila melanogaster, Drosophila melanogaster - genetics, Drosophila melanogaster - metabolism, Enzymes, Epidemiology, Flies, Gene Expression, Genetic engineering, Health risks, Heart attacks, High density lipoprotein, Hypotheses, Hypoxia - genetics, Hypoxia - metabolism, Hypoxia - mortality, Injury prevention, Insects, Internal medicine, Lipid peroxidation, Lipids, Lipoproteins (high density), Lipoproteins (low density), Low density lipoprotein, Low density lipoproteins, Male, Medical research, Medicine, Oxidative stress, Oxidative Stress - genetics, Paraoxonase, Paraoxonase 1, Polymorphism, Genetic, Prevention, Proteins, Reactive Oxygen Species - metabolism, Rodents, Stroke, Survival, Systematic review, Transgenic