PloS one, 2013-12, Vol.8 (12), p.e83011-e83011
2013
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Details
- Autor(en) / Beteiligte
- Titel
- OsDREB2A, a rice transcription factor, significantly affects salt tolerance in transgenic soybean
- Ist Teil von
- PloS one, 2013-12, Vol.8 (12), p.e83011-e83011
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The dehydration responsive element binding (DREB) transcription factors play an important role in regulating stress-related genes. OsDREB2A, a member of the DREBP subfamily of AP2/ERF transcription factors in rice (Oryza sativa), is involved in the abiotic stress response. OsDREB2A expression is induced by drought, low-temperature and salt stresses. Here, we report the ability of OsDREB2A to regulate high-salt response in transgenic soybean. Overexpressing OsDREB2A in soybeans enhanced salt tolerance by accumulating osmolytes, such as soluble sugars and free proline, and improving the expression levels of some stress-responsive transcription factors and key genes. The phenotypic characterization of transgenic soybean were significantly better than those of wild-type (WT). Electrophoresis mobility shift assay (EMSA) revealed that the OsDREB2A can bind to the DRE core element in vitro. These results indicate that OsDREB2A may participate in abiotic stress by directly binding with DRE element to regulate the expression of downstream genes. Overexpression of OsDREB2A in soybean might be used to improve tolerance to salt stress.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0083011Titel-ID: cdi_plos_journals_1470079207
- Format
- –
- Schlagworte
- Abiotic stress, Agriculture, Arabidopsis, Binding, Biosynthesis, Brassica, Cloning, Cold Temperature, Dehydration, DNA binding proteins, Drought, Electrophoretic mobility, Electrophoretic Mobility Shift Assay, Gene expression, Gene Expression Regulation, Plant, Genes, Genetic aspects, Genetic engineering, Genetically engineered foods, Genetically modified crops, Glycine max, Glycine max - drug effects, Glycine max - genetics, Glycine max - metabolism, Laboratories, Low temperature, Oryza - drug effects, Oryza - genetics, Oryza - metabolism, Oryza sativa, Plant Proteins - genetics, Plant Proteins - metabolism, Plants, Genetically Modified, Proline, Protein Binding, Regulatory sequences, Response Elements, Rice, Salinity, Salinity tolerance, Salt tolerance, Salt Tolerance - genetics, Salts, Seeds, Signal transduction, Sodium Chloride - pharmacology, Soybeans, Stress, Stress response, Stress, Physiological - genetics, Stresses, Sugar, Transcription factors, Transcription Factors - genetics, Transcription Factors - metabolism