PloS one, 2013-12, Vol.8 (12), p.e80931-e80931
2013
Details
- Autor(en) / Beteiligte
- Titel
- Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-α production in RBL-2H3 cells
- Ist Teil von
- PloS one, 2013-12, Vol.8 (12), p.e80931-e80931
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- Tumor necrosis factor alpha (TNF-α), a central mediator of the inflammatory response, is released from basophilic cells and other cells in response to a variety of proinflammatory stimuli. Vialinin A is a potent inhibitor of TNF-α production and is released from RBL-2H3 cells. Ubiquitin-specific peptidase 5 (USP5), a deubiquitinating enzyme, was identified as a target molecule of vialinin A and its enzymatic activity was inhibited by vialinin A. Here we report production of TNF-α is decreased in USP5 siRNA-knockdown RBL-2H3 cells, compared with control cells. The finding of the present study strongly suggests that USP5 is one of the essential molecules for the production of TNF-α in RBL-2H3.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0080931Titel-ID: cdi_plos_journals_1464982462
- Format
- –
- Schlagworte
- Animals, Antigens, Blotting, Western, Cell culture, Cell Line, Crohns disease, Cytokines, Endopeptidases - metabolism, Enzymatic activity, Enzymes, Experiments, Gene expression, Inflammation, Inflammatory response, Interleukin-4 - metabolism, Leukemia, Peptidase, Proteins, Rats, Reverse Transcriptase Polymerase Chain Reaction, Rheumatoid arthritis, RNA, Small Interfering, Science, siRNA, Stem cells, Target recognition, Terphenyl Compounds - metabolism, Tumor Necrosis Factor-alpha - metabolism, Tumor necrosis factor-TNF, Tumor necrosis factor-α, Ubiquitin