PloS one, 2013-11, Vol.8 (11), p.e81125-e81125
2013
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Details
- Autor(en) / Beteiligte
- Titel
- Targeted selected reaction monitoring mass spectrometric immunoassay for insulin-like growth factor 1
- Ist Teil von
- PloS one, 2013-11, Vol.8 (11), p.e81125-e81125
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Insulin-like growth factor 1 (IGF1) is an important biomarker of human growth disorders that is routinely analyzed in clinical laboratories. Mass spectrometry-based workflows offer a viable alternative to standard IGF1 immunoassays, which utilize various pre-analytical preparation strategies. In this work we developed an assay that incorporates a novel sample preparation method for dissociating IGF1 from its binding proteins. The workflow also includes an immunoaffinity step using antibody-derivatized pipette tips, followed by elution, trypsin digestion, and LC-MS/MS separation and detection of the signature peptides in a selected reaction monitoring (SRM) mode. The resulting quantitative mass spectrometric immunoassay (MSIA) exhibited good linearity in the range of 1 to 1,500 ng/mL IGF1, intra- and inter-assay precision with CVs of less than 10%, and lowest limits of detection of 1 ng/mL. The linearity and recovery characteristics of the assay were also established, and the new method compared to a commercially available immunoassay using a large cohort of human serum samples. The IGF1 SRM MSIA is well suited for use in clinical laboratories.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0081125Titel-ID: cdi_plos_journals_1460504788
- Format
- –
- Schlagworte
- Analysis, Antibodies, Automation, Binding proteins, Biomarkers, Biomarkers - blood, Biomedical laboratories, Elution, Health care networks, Heart failure, Humans, Immunoassay, Immunoassay - methods, Immunoassay - standards, Immunoassays, Insulin, Insulin-like growth factor I, Insulin-Like Growth Factor I - chemistry, Insulin-Like Growth Factor I - metabolism, Insulin-like growth factors, Laboratories, Linearity, Mass spectrometry, Mass Spectrometry - methods, Mass Spectrometry - standards, Mass spectroscopy, Methods, Monitoring, Peptides, Plasma, Protein binding, Proteins, Proteomics, Reference Standards, Reference Values, Reproducibility of Results, Sample preparation, Scientific imaging, Sensitivity and Specificity, Studies, Tips, Trypsin, Workflow