PloS one, 2013-07, Vol.8 (7), p.e71291-e71291
2013
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- Autor(en) / Beteiligte
- Titel
- Immature dendritic cells generated from cryopreserved human monocytes show impaired ability to respond to LPS and to induce allogeneic lymphocyte proliferation
- Ist Teil von
- PloS one, 2013-07, Vol.8 (7), p.e71291-e71291
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Dendritic cells play a key role in the immune system, in the sensing of foreign antigens and triggering of an adaptive immune response. Cryopreservation of human monocytes was investigated to understand its effect on differentiation into immature monocyte-derived dendritic cells (imdDCs), the response to inflammatory stimuli and the ability to induce allogeneic lymphocyte proliferation. Cryopreserved (crp)-monocytes were able to differentiate into imdDCs, albeit to a lesser extent than freshly (frh)-obtained monocytes. Furthermore, crp-imdDCs had lower rates of maturation and cytokine/chemokine secretion in response to LPS than frh-imdDCs. Lower expression of Toll-like receptor 4 (at 24 and 48 h) and higher susceptibility to apoptosis in crp-imdDCs than in fresh cells would account for the impaired maturation and cytokine/chemokine secretion observed. A mixed leukocyte reaction showed that lymphocyte proliferation was lower with crp-imdDCs than with frh-imdDCs. These findings suggested that the source of monocytes used to generate human imdDCs could influence the accuracy of results observed in studies of the immune response to pathogens, lymphocyte activation, vaccination and antigen sensing. It is not always possible to work with freshly isolated monocytes but the possible effects of freezing/thawing on the biology and responsiveness of imdDCs should be taken into account.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0071291Titel-ID: cdi_plos_journals_1440992428
- Format
- –
- Schlagworte
- Adaptive immunity, Adaptive systems, Antigens, Apoptosis, Apoptosis - drug effects, Apoptosis - immunology, Autoimmune diseases, Biological effects, Biology, Cell activation, Cell Differentiation - drug effects, Cell Differentiation - immunology, Cell proliferation, Cell Proliferation - drug effects, Cells, Cultured, Chemokines, Chemokines - immunology, Chemokines - metabolism, Cryopreservation, Cryopreservation - methods, Cytokines, Cytokines - immunology, Cytokines - metabolism, Dendritic cells, Dendritic Cells - drug effects, Dendritic Cells - immunology, Dendritic Cells - metabolism, Flow Cytometry, Freezing, Freezing point, Granulocyte-Macrophage Colony-Stimulating Factor - immunology, Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology, Human behavior, Human performance, Humans, Immune response, Immune system, Immunoglobulins, Immunology, Inflammation, Interleukin-4 - immunology, Interleukin-4 - pharmacology, Leukocytes, Lipopolysaccharide Receptors - immunology, Lipopolysaccharide Receptors - metabolism, Lipopolysaccharides, Lipopolysaccharides - immunology, Lipopolysaccharides - pharmacology, Lymphocyte Culture Test, Mixed - methods, Lymphocytes, Lymphocytes - drug effects, Lymphocytes - immunology, Lymphocytes - metabolism, Maturation, Mixed leukocyte reaction, Monocytes, Monocytes - drug effects, Monocytes - immunology, Monocytes - metabolism, Proteins, Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - immunology, Receptors, Granulocyte-Macrophage Colony-Stimulating Factor - metabolism, Receptors, Interleukin-4 - immunology, Receptors, Interleukin-4 - metabolism, Studies, Thawing, TLR4 protein, Toll-Like Receptor 4 - immunology, Toll-Like Receptor 4 - metabolism, Toll-like receptors, Vaccination