Details

Autor(en) / Beteiligte

• Schmitt, Sabrina
• Safferling, Kai
• Westphal, Kathi
• Hrabowski, Manuel
• Müller, Ute
• Angel, Peter
• Wiechert, Lars
• Ehemann, Volker
• Müller, Benedikt
• Holland-Cunz, Stefan
• Stichel, Damian
• Harder, Nathalie
• Rohr, Karl
• Germann, Günter
• Matthäus, Franziska
• Schirmacher, Peter
• Grabe, Niels
• Breuhahn, Kai
• Egles, Christophe

Titel

Stathmin regulates keratinocyte proliferation and migration during cutaneous regeneration

Ist Teil von

• PloS one, 2013-09, Vol.8 (9), p.e75075-e75075

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2013

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Cutaneous regeneration utilizes paracrine feedback mechanisms to fine-tune the regulation of epidermal keratinocyte proliferation and migration. However, it is unknown how fibroblast-derived hepatocyte growth factor (HGF) affects these mutually exclusive processes in distinct cell populations. We here show that HGF stimulates the expression and phosphorylation of the microtubule-destabilizing factor stathmin in primary human keratinocytes. Quantitative single cell- and cell population-based analyses revealed that basal stathmin levels are important for the migratory ability of keratinocytes in vitro; however, its expression is moderately induced in the migration tongue of mouse skin or organotypic multi-layered keratinocyte 3D cultures after full-thickness wounding. In contrast, clearly elevated stathmin expression is detectable in hyperproliferative epidermal areas. In vitro, stathmin silencing significantly reduced keratinocyte proliferation. Automated quantitative and time-resolved analyses in organotypic cocultures demonstrated a high correlation between Stathmin/phospho-Stathmin and Ki67 positivity in epidermal regions with proliferative activity. Thus, activation of stathmin may stimulate keratinocyte proliferation, while basal stathmin levels are sufficient for keratinocyte migration during cutaneous regeneration.