PloS one, 2013-08, Vol.8 (8), p.e70881
2013
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Details
- Autor(en) / Beteiligte
- Titel
- Neurotoxicity of prion peptides mimicking the central domain of the cellular prion protein
- Ist Teil von
- PloS one, 2013-08, Vol.8 (8), p.e70881
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The physiological functions of PrP(C) remain enigmatic, but the central domain, comprising highly conserved regions of the protein may play an important role. Indeed, a large number of studies indicate that synthetic peptides containing residues 106-126 (CR) located in the central domain (CD, 95-133) of PrP(C) are neurotoxic. The central domain comprises two chemically distinct subdomains, the charge cluster (CC, 95-110) and a hydrophobic region (HR, 112-133). The aim of the present study was to establish the individual cytotoxicity of CC, HR and CD. Our results show that only the CD peptide is neurotoxic. Biochemical, Transmission Electron Microscopy and Atomic Force Microscopy experiments demonstrated that the CD peptide is able to activate caspase-3 and disrupt the cell membrane, leading to cell death.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0070881Titel-ID: cdi_plos_journals_1430170854
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Apoptosis, Atomic force microscopy, Benzothiazoles, Bioengineering, Biology, Biomedical materials, Caspase, Caspase 3 - metabolism, Caspase-3, Cell death, Cell Membrane Permeability - drug effects, Cells, Cultured, Creutzfeldt-Jakob disease, Cytotoxicity, Dimyristoylphosphatidylcholine - chemistry, Electron microscopy, Enzyme Activation, Fluorescent Dyes - chemistry, Gene expression, Hydrophobicity, Kinetics, Lipid Bilayers - chemistry, Mathematics, Medicine, Mice, Mice, 129 Strain, Mice, Inbred C57BL, Mice, Knockout, Microscopy, Mimicry, Molecular Mimicry, Molecular Sequence Data, Mort cel·lular, Neurodegeneration, Neurons - drug effects, Neurons - physiology, Neurophysiology, Neurotoxicity, Peptide Fragments - chemistry, Peptide Fragments - pharmacology, Peptide Fragments - physiology, Peptide synthesis, Peptides, Physiology, Primary Cell Culture, Prion protein, Prions, Prions (Proteins), Protein Multimerization, Protein Structure, Tertiary, Proteins, Proteïnes, PrPC Proteins - chemistry, PrPC Proteins - pharmacology, PrPC Proteins - physiology, Pèptids, Studies, Synthetic peptides, Síntesi de pèptids, Thiazoles - chemistry, Toxicity, Transmission electron microscopy