PloS one, 2013-07, Vol.8 (7), p.e69151
2013
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- Autor(en) / Beteiligte
- Titel
- Effect of epicatechin against radiation-induced oral mucositis: in vitro and in vivo study
- Ist Teil von
- PloS one, 2013-07, Vol.8 (7), p.e69151
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Radiation-induced oral mucositis limits the delivery of high-dose radiation to head and neck cancer. This study investigated the effectiveness of epicatechin (EC), a component of green tea extracts, on radiation-induced oral mucositis in vitro and in vivo. The effect of EC on radiation-induced cytotoxicity was analyzed in the human keratinocyte line HaCaT. Radiation-induced apoptosis, change in mitochondrial membrane potential (MMP), reactive oxygen species (ROS) generation and changes in the signaling pathway were investigated. In vivo therapeutic effects of EC for oral mucositis were explored in a rat model. Rats were monitored by daily inspections of the oral cavity, amount of oral intake, weight change and survival rate. For histopathologic evaluation, hematoxylin-eosin staining and TUNEL staining were performed. EC significantly inhibited radiation-induced apoptosis, change of MMP, and intracellular ROS generation in HaCaT cells. EC treatment markedly attenuated the expression of p-JNK, p-38, and cleaved caspase-3 after irradiation in the HaCaT cells. Rats with radiation-induced oral mucositis showed decreased oral intake, weight and survival rate, but oral administration of EC significantly restored all three parameters. Histopathologic changes were significantly decreased in the EC-treated irradiated rats. TUNEL staining of rat oral mucosa revealed that EC treatment significantly decreased radiation-induced apoptotic cells. This study suggests that EC significantly inhibited radiation-induced apoptosis in keratinocytes and rat oral mucosa and may be a safe and effective candidate treatment for the prevention of radiation-induced mucositis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0069151Titel-ID: cdi_plos_journals_1427370162
- Format
- –
- Schlagworte
- Analysis, Analysis of Variance, Animals, Apoptosis, Apoptosis - drug effects, Biocompatibility, Biology, Blotting, Western, Cancer, Caspase, Caspase-3, Catechin - pharmacology, Catechin - therapeutic use, Cytotoxicity, Epicatechin, Experimental design, Female, Fluorescein-5-isothiocyanate, Green tea, Head, Head & neck cancer, Head and neck cancer, Head and Neck Neoplasms - radiotherapy, Health aspects, Humans, Immunohistochemistry, In Situ Nick-End Labeling, In vivo methods and tests, Irradiation, JNK protein, Keratinocytes, Keratinocytes - drug effects, Kinases, Medicine, Membrane potential, Membrane Potential, Mitochondrial - drug effects, Microscopy, Fluorescence, Mitochondria, Mucosa, Mucositis, Oral administration, Oral cavity, Otolaryngology, Oxygen, Physics, Propidium, Radiation, Radiation (Physics), Radiation dosage, Radiation effects, Radiotherapy - adverse effects, Rats, Rats, Sprague-Dawley, Reactive oxygen species, Reactive Oxygen Species - metabolism, Signal transduction, Signal Transduction - drug effects, Signaling, Staining, Stomatitis, Stomatitis - drug therapy, Stomatitis - etiology, Survival, Tea, Toxicity