PloS one, 2013-04, Vol.8 (4), p.e61135
2013
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- Autor(en) / Beteiligte
- Titel
- Enhanced immune response and protective effects of nano-chitosan-based DNA vaccine encoding T cell epitopes of Esat-6 and FL against Mycobacterium tuberculosis infection
- Ist Teil von
- PloS one, 2013-04, Vol.8 (4), p.e61135
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Development of a novel and effective vaccine against Mycobacterium tuberculosis (M.tb) is a challenging for preventing TB infection. In this study, a novel nanoparticle-based recombinant DNA vaccine was developed, which contains Esat-6 three T cell epitopes (Esat-6/3e) and fms-like tyrosine kinase 3 ligand (FL) genes (termed Esat-6/3e-FL), and was enveloped with chitosan (CS) nanoparticles (nano-chitosan). The immunologic and protective efficacy of the nano-chitosan-based DNA vaccine (termed nano-Esat-6/3e-FL) was assessed in C57BL/6 mice after intramuscular prime vaccination with the plasmids DNA and nasal boost with the Esat-6/3e peptides. The results showed that the immunized mice remarkably elicited enhanced T cell responses and protection against M.tb H37Rv challenge. These findings indicate that the nano-chitosan can significantly elevate the immunologic and protective effects of the DNA vaccine, and the nano-Esat-6/3e-FL is a useful vaccine for preventing M.tb infection in mice.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0061135Titel-ID: cdi_plos_journals_1346594967
- Format
- –
- Schlagworte
- Amino Acid Sequence, Analysis, Animals, Antigenic determinants, Antigens, Antigens, Bacterial - chemistry, Antigens, Bacterial - genetics, B cells, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Biology, Cell Count, Cell Proliferation, Chitosan, Chitosan - chemistry, Cytokines - biosynthesis, Cytotoxicity, Dendritic cells, Deoxyribonucleic acid, DNA, DNA vaccines, Epitopes, Epitopes, T-Lymphocyte - immunology, ESAT-6 antigen, Female, Flt3 protein, fms-Like Tyrosine Kinase 3 - metabolism, GATA3 Transcription Factor - metabolism, Gene Expression Regulation, Health aspects, Identification, Immune response, Immune system, Immunization, Immunology, Infections, Interferon-gamma - metabolism, Ligands, Lymphocytes, Lymphocytes T, Materials Science, Medical research, Medicine, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Mycobacterium tuberculosis, Mycobacterium tuberculosis - immunology, Mycobacterium tuberculosis - physiology, Nanoparticles, Nanoparticles - chemistry, Particle Size, Peptides, Plasmids, Protein-tyrosine kinase, Proteins, Recombinant DNA, Recombinant Proteins - chemistry, Recombinant Proteins - genetics, Spleen - cytology, T cells, T-Box Domain Proteins - metabolism, T-Lymphocytes, Cytotoxic - cytology, T-Lymphocytes, Cytotoxic - immunology, T-Lymphocytes, Cytotoxic - metabolism, Transfection, Tuberculosis, Tuberculosis - prevention & control, Tuberculosis vaccines, Tyrosine, Vaccination, Vaccines, Vaccines, DNA - genetics, Vaccines, DNA - immunology