Details
- Autor(en) / Beteiligte
- Titel
- The mode of action of isocyanide in three aquatic organisms, Balanus amphitrite, Bugula neritina and Danio rerio
- Ist Teil von
- PloS one, 2012-09, Vol.7 (9), p.e45442
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Isocyanide is a potential antifouling compound in marine environments. In this study, we investigated its mode of action in three aquatic organisms. Two of them, the bryozoan Bugula neritina and the barnacle Balanus amphitrite, are major marine fouling invertebrates, and the other organism is the non-target species zebrafish Danio rerio. In the swimming larvae of B. neritina, isocyanide did not affect the total attachment rate (≤50 µg ml(-1)), but it did change the attachment site by increasing the percentage of attachment on the bottom of the container rather than on the wall or air-water inter-surface. Isocyanide binds several proteins in B. neritina as identified via SDS-PAGE-LC-MS/MS: 1) a 30 kD protein band containing two proteins similar to voltage dependent anion channels (VDAC), which control the direct coupling of the mitochondrial matrix to the energy maintenance of the cytosol and the release of apoptogenic factors from mitochondria of mammalian cells; and 2) an unknown 39 kD protein. In B. amphitrite cyprids, the isocyanide binding protein were 1) a protein similar to NADH-ubiquinone oxidoreductase, which is the "entry enzyme" of oxidative phosphorylation in mitochondria; and 2) cytochrome P450. In Danio rerio embryos, isocyanide caused "wavy" notochords, hydrocephalus, pericardial edema, poor blood circulation, and defects in pigmentation and hematopoiesis, which phenocopied copper deficiency. This is the first report on isocyanide binding proteins in fouling organisms, as well as the first description of its phenotype and potential toxicology in zebrafish.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0045442Titel-ID: cdi_plos_journals_1344508551
- Format
- –
- Schlagworte
- Animals, Anion channels, Antifouling, Antifouling substances, Aquatic organisms, Aquatic Organisms - drug effects, Aquatic Organisms - metabolism, Attachment, Balanus amphitrite, Binding, Biochemistry, Biofouling, Biology, Blood circulation, Bryozoa - drug effects, Bryozoa - metabolism, Bugula neritina, Chemical compounds, Collaboration, Containers, Copper, Cyanides, Cyanides - toxicity, Cytochrome, Cytochrome P450, Cytosol, Danio rerio, Edema, Embryos, Fouling, Fouling organisms, Gel electrophoresis, Hematopoiesis, Hemopoiesis, Histology, Hydrocephalus, Invertebrates, Laboratories, Larvae, Life sciences, Mammalian cells, Marine environment, Marine invertebrates, Metabolism, Mitochondria, Mode of action, NADH, NADH-ubiquinone oxidoreductase, Nicotinamide adenine dinucleotide, Organisms, Oxidative phosphorylation, Oxidoreductase, Phosphorylation, Physiological aspects, Pigmentation, Proteins, Sodium lauryl sulfate, Swimming, Thoracica - drug effects, Thoracica - metabolism, Toxicology, Ubiquinone, Ubiquinone oxidoreductase, Zebrafish, Zebrafish - metabolism