PloS one, 2012-11, Vol.7 (11), p.e49234-e49234
2012
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- Autor(en) / Beteiligte
- Titel
- Schistosoma japonicum soluble egg antigens attenuate IFN-γ-induced MHC class II expression in RAW 264.7 macrophages
- Ist Teil von
- PloS one, 2012-11, Vol.7 (11), p.e49234-e49234
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Innate immune response plays the key role in initiating and guiding the immune response. Elucidating the innate immune related molecular events involved in the interaction between the parasite and the host will aid in the development of an effective vaccine and anti-schistosome pharmaceuticals. In this study, we examined the regulatory effect of Schistosoma japonicum soluble egg antigen (SEA) on MHC class II expression in macrophage cell line RAW 264.7. We demonstrated that SEA possesses the ability to down-regulate IFN-γ-induced MHC class II expression in RAW 264.7 cells. The production of IL-10 and IL-6 in RAW 264.7 cells, induced by SEA, was responsible for mediating the down-regulation of MHC class II. Our findings suggest that in RAW 264.7 cells (1) IFN-γ provides a condition for lower concentrations of SEA to attenuate MHC class II expression; (2) SEA attenuated IFN-γ-induced MHC class II expression and the IL-10 and IL-6 production is mediated at least partly by the interaction of SEA with TLR4; and (3) SEA attenuated IFN-γ-induced MHC class II expression at the transcriptional level.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0049234Titel-ID: cdi_plos_journals_1335102066
- Format
- –
- Schlagworte
- Animals, Antigen presentation, Antigens, Antigens, Helminth - immunology, Attenuation, Biology, Cells, Cultured, Cytokines, Drugs, Eggs, Gene expression, Histocompatibility Antigens Class II - metabolism, Immune response, Immune system, Immunology, Infections, Innate immunity, Interferon, Interferon-gamma - pharmacology, Interleukin 10, Interleukin 6, Interleukin-10 - biosynthesis, Interleukin-6 - biosynthesis, Laboratories, Lymphocytes, Macrophages, Macrophages, Peritoneal - drug effects, Macrophages, Peritoneal - immunology, Major histocompatibility complex, Medicine, Mice, Mice, Inbred C57BL, Mortality, Ovum - drug effects, Ovum - immunology, Parasitic diseases, Pathogens, Rabbits, Schistosoma japonicum, Schistosoma japonicum - immunology, Soluble egg antigen, Studies, TLR4 protein, Toll-Like Receptor 4 - metabolism, Toll-like receptors, Transcription, Tropical diseases, γ-Interferon