Details

Autor(en) / Beteiligte

• Enoki, Sawako
• Iino, Ryota
• Morone, Nobuhiro
• Kaihatsu, Kunihiro
• Sakakihara, Shouichi
• Kato, Nobuo
• Noji, Hiroyuki
• Digard, Paul

Titel

Label-free single-particle imaging of the influenza virus by objective-type total internal reflection dark-field microscopy

Ist Teil von

• PloS one, 2012-11, Vol.7 (11), p.e49208-e49208

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2012

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Here we report label-free optical imaging of single particles of the influenza virus attached on a glass surface with a simple objective-type total internal reflection dark-field microscopy (TIRDFM). The capability of TIRDFM for the imaging of single viral particles was confirmed from fine correlation of the TIRDFM images with fluorescent immunostaining image and scanning electron microscopy image. The density of scattering spots in the TIRDFM images showed a good linearity against the virus concentration, giving the limit of detection as 1.2×10(4) plaque-forming units per milliliter. Our label-free optical imaging method does require neither elaborated sample preparation nor complex optical systems, offering a good platform for rapid and sensitive counting of viral particles.