PloS one, 2013-04, Vol.8 (4), p.e59364
2013
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Details
- Autor(en) / Beteiligte
- Titel
- The influence of non polar and polar molecules in mouse motile cells membranes and pure lipid bilayers
- Ist Teil von
- PloS one, 2013-04, Vol.8 (4), p.e59364
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We report an experimental study of mouse sperm motility that shows chief aspects characteristic of neurons: the anesthetic (produced by tetracaine) and excitatory (produced by either caffeine or calcium) effects and their antagonic action. While tetracaine inhibits sperm motility and caffeine has an excitatory action, the combination of these two substances balance the effects, producing a motility quite similar to that of control cells. We also study the effects of these agents (anesthetic and excitatory) on the melting points of pure lipid liposomes constituted by 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and dipalmitoyl phosphatidic acid (DPPA). Tetracaine induces a large fluidization of the membrane, shifting the liposomes melting transition temperature to much lower values. The effect of caffeine is null, but its addition to tetracaine-doped liposomes greatly screen the fluidization effect. A high calcium concentration stiffens pure lipid membranes and strongly reduces the effect of tetracaine. Molecular Dynamics Simulations are performed to further understand our experimental findings at the molecular level. We find a strong correlation between the effect of antagonic molecules that could explain how the mechanical properties suitable for normal cell functioning are affected and recovered.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0059364Titel-ID: cdi_plos_journals_1330900723
- Format
- –
- Schlagworte
- Anesthesia, Animals, Binding sites, Biology, Biophysics, Caffeine, Caffeine - pharmacology, Calcium, Calcium content, Cell Membrane - drug effects, Cell Membrane - metabolism, Chemistry, Drugs, Fertility, Fluidization, Fluidizing, Genetic aspects, Hydrophobic and Hydrophilic Interactions, Kinases, Lipid Bilayers, Lipid membranes, Lipids, Liposomes, Liposomes - chemistry, Liposomes - metabolism, Male, Mechanical properties, Melting, Melting points, Membrane Lipids - chemistry, Membrane Lipids - metabolism, Membranes, Metabolism, Mice, Molecular Conformation, Molecular dynamics, Molecular Dynamics Simulation, Molecular structure, Motility, Narcotics, Neurons, Phosphatidic acid, Phosphocholine, Physics, Proteins, Sperm, Sperm Motility - drug effects, Sperm Motility - physiology, Spermatozoa - drug effects, Spermatozoa - metabolism, Studies, Temperature, Temperature effects, Tetracaine, Tetracaine - pharmacology, Transition temperature, Transition temperatures