Details

Autor(en) / Beteiligte

• De, Debashree
• Datta Chakraborty, Piyali
• Mitra, Jyotirmoy
• Sharma, Kanika
• Mandal, Somnath
• Das, Aneesha
• Chakrabarti, Saikat
• Bhattacharyya, Debasish
• Yang, Chuen-Mao

Titel

Ubiquitin-like protein from human placental extract exhibits collagenase activity

Ist Teil von

• PloS one, 2013-03, Vol.8 (3), p.e59585-e59585

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• An aqueous extract of human placenta exhibits strong gelatinase/collagenase activity in zymography. 2-D gel electrophoresis of the extract with gelatin zymography in the second dimension displayed a single spot, identified as ubiquitin-like component upon MALDI/TOF MS/MS analysis. Immunoblot indicated presence of ubiquitin and absence of collagenase in the extract. Collagenase activity of the ubiquitin-like component was confirmed from the change in solubility of collagen in aqueous buffer, degradation of collagen by size-exclusion HPLC and atomic force microscopy. Quantification with DQ-gelatin showed that the extract contains 0.04 U/ml of collagenase activity that was inhibited up to 95% by ubiquitin antibody. Ubiquitin from bovine erythrocytes demonstrated mild collagenase activity. Bioinformatics studies suggest that placental ubiquitin and collagenase follow structurally divergent evolution. This thermostable intrinsic collagenase activity of placental extract might have wide physiological relevance in degrading and remodeling collagen as it is used as a drug for wound healing and pelvic inflammatory diseases.