PloS one, 2013-03, Vol.8 (3), p.e59131
2013
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- Autor(en) / Beteiligte
- Titel
- Integrated analysis of COX-2 and iNOS derived inflammatory mediators in LPS-stimulated RAW macrophages pre-exposed to Echium plantagineum L. bee pollen extract
- Ist Teil von
- PloS one, 2013-03, Vol.8 (3), p.e59131
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Oxidative stress and inflammation play important roles in disease development. This study intended to evaluate the anti-inflammatory and antioxidant potential of Echium plantagineum L. bee pollen to support its claimed health beneficial effects. The hydromethanol extract efficiently scavenged nitric oxide ((•)NO) although against superoxide (O2(•-)) it behaved as antioxidant at lower concentrations and as pro-oxidant at higher concentrations. The anti-inflammatory potential was evaluated in LPS-stimulated macrophages. The levels of (•)NO and L-citrulline decreased for all extract concentrations tested, while the levels of prostaglandins, their metabolites and isoprostanes, evaluated by UPLC-MS, decreased with low extract concentrations. So, E. plantagineum bee pollen extract can exert anti-inflammatory activity by reducing (•)NO and prostaglandins. The extract is able to scavenge the reactive species (•)NO and O2(•-) and reduce markers of oxidative stress in cells at low concentrations.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0059131Titel-ID: cdi_plos_journals_1330882355
- Format
- –
- Schlagworte
- Amino acids, Analysis, Animals, Antiinflammatory agents, Antioxidants, Antioxidants (Nutrients), Bees, Biological activity, Biology, Biosynthesis, Cell Line, Chemical compounds, Chemistry, Citrulline, Citrulline - metabolism, Complex Mixtures - chemistry, Complex Mixtures - pharmacology, Cyclooxygenase 2 - metabolism, Cyclooxygenase-2, Cytochrome, Development and progression, Echium - chemistry, Echium plantagineum, Enzymes, Flavonoids, Flowers & plants, Food science, Free Radical Scavengers - chemistry, Free Radical Scavengers - pharmacology, Gene expression, Health aspects, Inflammation, Inflammation Mediators - metabolism, Isoprostanes, L-citrulline, Lipopolysaccharides, Low concentrations, Macrophages, Macrophages - metabolism, Metabolites, Mice, Nitric oxide, Nitric Oxide - metabolism, Nitric Oxide Synthase Type II - metabolism, Nitric-oxide synthase, Oxidative stress, Pollen, Pollen - chemistry, Prostaglandins, Rodents, Superoxide, Superoxides - metabolism