Details

Autor(en) / Beteiligte

• Charpentier, Charlotte
• Lambert-Niclot, Sidonie
• Alteri, Claudia
• Storto, Alexandre
• Flandre, Philippe
• Svicher, Valentina
• Perno, Carlo-Federico
• Brun-Vézinet, Françoise
• Calvez, Vincent
• Marcelin, Anne-Geneviève
• Ceccherini-Silberstein, Francesca
• Descamps, Diane
• Menéndez-Arias, Luis

Titel

Description of the L76V resistance protease mutation in HIV-1 B and "non-B" subtypes

Ist Teil von

• PloS one, 2013-01, Vol.8 (1), p.e54381-e54381

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2013

Quelle

Elektronische Zeitschriftenbibliothek (Open access)

Beschreibungen/Notizen

• To describe the prevalence of the L76V protease inhibitors resistance-associated mutation (PI-RAM) in relation with patients' characteristics and protease genotypic background in HIV-1 B- and "non-B"-infected patients. Frequency of the L76V mutation between 1998 and 2010 was surveyed in the laboratory database of 3 clinical centers. Major PI-RAMs were identified according to the IAS-USA list. Fisher's and Wilcoxon tests were used to compare variables. Among the overall 29,643 sequences analyzed, the prevalence of L76V was 1.50%, while was 5.42% in PI-resistant viruses. Since 2008 the prevalence of L76V was higher in "non-B"-infected than in B-infected patients each year. Median time since diagnosis of HIV-1 infection and median time under antiretroviral-based regimen were both shorter in "non-B"- than in B-infected patients (8 vs 11 years, P<0.0001; and 7 vs 8 years, P = 0.004). In addition, "non-B"-infected patients had been pre-exposed to a lower number of PI (2 vs 3, P = 0.016). The L76V was also associated with a lower number of major PI-RAMs in "non-B" vs B samples (3 vs 4, P = 0.0001), and thus it was more frequent found as single major PI-RAM in "non-B" vs B subtype (10% vs 2%, P = 0.014). We showed an impact of viral subtype on the selection of the L76V major PI-RAM with a higher prevalence in "non-B" subtypes observed since 2008. In addition, in "non-B"-infected patients this mutation appeared more rapidly and was associated with less PI-RAM.