Details

Autor(en) / Beteiligte

• Palacín, Arantxa
• Gómez-Casado, Cristina
• Rivas, Luis A
• Aguirre, Jacobo
• Tordesillas, Leticia
• Bartra, Joan
• Blanco, Carlos
• Carrillo, Teresa
• Cuesta-Herranz, Javier
• de Frutos, Consolación
• Alvarez-Eire, Genoveva García
• Fernández, Francisco J
• Gamboa, Pedro
• Muñoz, Rosa
• Sánchez-Monge, Rosa
• Sirvent, Sofía
• Torres, María J
• Varela-Losada, Susana
• Rodríguez, Rosalía
• Parro, Victor
• Blanca, Miguel
• Salcedo, Gabriel
• Díaz-Perales, Araceli
• Stumbles, Phillip A.

Titel

Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study

Ist Teil von

• PloS one, 2012-12, Vol.7 (12), p.e50799-e50799

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.