Details
- Autor(en) / Beteiligte
- Titel
- IL-21 regulates the differentiation of a human γδ T cell subset equipped with B cell helper activity
- Ist Teil von
- PloS one, 2012-07, Vol.7 (7), p.e41940
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Vγ9Vδ2 T lymphocytes recognize nonpeptidic antigens without presentation by MHC molecules and display pleiotropic features. Here we report that coculture of Vγ9Vδ2 cells with phosphoantigen and IL-21 leads to selective expression of the transcription repressor Bcl-6 and polarization toward a lymphocyte subset displaying features of follicular B-helper T (T(FH)) cells. T(FH)-like Vγ9Vδ2 cells have a predominant central memory (CD27(+)CD45RA(-)) phenotype and express ICOS, CD40L and CXCR5. Upon antigen activation, they secrete IL-4, IL-10 and CXCL13, and provide B-cell help for antibody production in vitro. Our findings delineate a subset of human Vγ9Vδ2 lymphocytes, which, upon interaction with IL-21-producing CD4 T(FH) cells and B cells in secondary lymphoid organs, is implicated in the production of high affinity antibodies against microbial pathogens.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0041940Titel-ID: cdi_plos_journals_1327118608
- Format
- –
- Schlagworte
- Antibodies, Antibody Affinity - drug effects, Antigen presentation, Antigens, B-Lymphocytes - immunology, Bcl-6 protein, Biology, CD27 antigen, CD4 antigen, CD4 Antigens - metabolism, CD40L protein, CD45RA antigen, Cell Differentiation - drug effects, Cell Movement - drug effects, Cell Proliferation - drug effects, Chemokines - biosynthesis, CXCL13 protein, CXCR5 protein, Cytokines, Dendritic cells, DNA-Binding Proteins - metabolism, E coli, Escherichia coli, Gene Expression Regulation - drug effects, Humans, Immunoglobulin delta-Chains - metabolism, Immunoglobulin gamma-Chains - metabolism, Immunoglobulins, Immunological memory, Immunotherapy, Interleukin 10, Interleukin 21, Interleukin 4, Interleukins - pharmacology, Lymphocytes, Lymphocytes B, Lymphocytes T, Major histocompatibility complex, Medicine, Microorganisms, Organs, Proto-Oncogene Proteins c-bcl-6, Studies, T cell receptors, T-Lymphocytes, Helper-Inducer - cytology, T-Lymphocytes, Helper-Inducer - drug effects, T-Lymphocytes, Helper-Inducer - immunology, T-Lymphocytes, Helper-Inducer - metabolism, Transcription, Tuberculosis